Hotspot

rpl14-rpl16

LSC · 77238-77346 · 109 bp

Back to Schizachyrium

Pi 0.0203

MarkerSeek score 65.80

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 109
variable_sites: 12
pi: 0.0203
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	GATTACCAGCGGCTCCTATG	TAGCTGCCAGTAAAATGCCC	55.0	50.0	57.9	58.2	248-259 bp	1.0	83.4
rpl14-rpl16_p2	ATTACCAGCGGCTCCTATGA	TAGCTGCCAGTAAAATGCCC	50.0	50.0	58.3	58.2	247-258 bp	1.0	81.9
rpl14-rpl16_p3	TGATTACCAGCGGCTCCTAT	TAGCTGCCAGTAAAATGCCC	50.0	50.0	58.3	58.2	249-260 bp	1.0	81.8
rpl14-rpl16_p4	CGAGCTCCACTGTTATCTGC	TAGCTGCCAGTAAAATGCCC	55.0	50.0	58.4	58.2	207-218 bp	1.0	81.4
rpl14-rpl16_p5	GATTACCAGCGGCTCCTATG	GCCAGTAAAATGCCCATACG	55.0	50.0	57.9	57.2	243-254 bp	1.0	79.2