Hotspot

trnF-GAA-ndhJ

LSC · 48242-48690 · 449 bp

Back to Schizachyrium

Pi 0.0137

MarkerSeek score 58.50

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 449
variable_sites: 42
pi: 0.0137
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	548-691 bp	1.0	87.8
trnF-GAA-ndhJ_p2	ATGCACAAGAAAGGGTCAGG	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	601-744 bp	1.0	87.1
trnF-GAA-ndhJ_p3	GCACAAGAAAGGGTCAGGAT	ATAGGCTGGCCCTTACGTAA	50.0	50.0	57.8	58.2	599-742 bp	1.0	86.1
trnF-GAA-ndhJ_p4	AATCCTCGTGTCACCAGTTC	ATAGGCTGGCCCTTACGTAA	50.0	50.0	57.5	58.2	551-694 bp	1.0	84.5
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	GATAGGCTGGCCCTTACGTA	50.0	55.0	58.1	58.7	549-692 bp	1.0	84.1