Hotspot

ndhG

SSC · 110066-110596 · 531 bp

Back to Schizachne

Pi 0.00565

MarkerSeek score 47.60

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 3
pi: 0.00565
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	ACCATGTTTCGGCTTGTGAT	GAGTACTGTCCGACAAGCTG	45.0	55.0	58.1	58.0	1299-1300 bp	1.0	79.6
ndhG_p2	ACCATGTTTCGGCTTGTGAT	GTGTTCGCGTATGTCCGATA	45.0	50.0	58.1	57.9	1424-1425 bp	1.0	78.8
ndhG_p3	ACCATGTTTCGGCTTGTGAT	TGTGAAGTATGTGTTCGCGT	45.0	45.0	58.1	57.9	1434-1435 bp	1.0	78.5
ndhG_p4	ACCATGTTTCGGCTTGTGAT	GTACTGTCCGACAAGCTGTT	45.0	50.0	58.1	57.8	1297-1298 bp	1.0	78.5
ndhG_p5	ACCATGTTTCGGCTTGTGAT	ACCTCTCGTGGATTGGAGAT	45.0	50.0	58.1	57.8	1399-1400 bp	1.0	78.3