Hotspot

ndhF

SSC · 101956-104175 · 2220 bp

Back to Schizachne

Pi 0.0018
MarkerSeek score 45.50
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2220
variable_sites
4
pi
0.0018
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 GGAGACCCTAAATTCTTTACG TGGTTTGGTGAATCGGAAACA 42.9 42.9 53.7 58.3 2310 bp 1.0 40.1
ndhF_p2 GGAGACCCTAAATTCTTTACG GGTTTGGTGAATCGGAAACA 42.9 45.0 53.7 56.5 2309 bp 1.0 40.1
ndhF_p3 GGAGACCCTAAATTCTTTACG TGGTTTGGTGAATCGGAAAC 42.9 45.0 53.7 56.5 2310 bp 1.0 40.1
ndhF_p4 GGAGACCCTAAATTCTTTACG GGTTTGGTGAATCGGAAACAA 42.9 42.9 53.7 57.3 2309 bp 1.0 40.1
ndhF_p5 GGAGACCCTAAATTCTTTACG TTGGTTTGGTGAATCGGAAAC 42.9 42.9 53.7 57.3 2311 bp 1.0 40.1