Hotspot

ndhF-rpl32

SSC · 104176-105015 · 840 bp

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Pi 0.00719
MarkerSeek score 59.70
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
840
variable_sites
6
pi
0.00719
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAAGTGGGAGAAGAGGGAT TCCAAAGATTTTTACGAATACGCT 55.0 33.3 58.1 57.3 948-951 bp 1.0 42.3
ndhF-rpl32_p2 GGAAGTGGGAGAAGAGGGAT TGACATTGAGGTACGTTTTT 55.0 35.0 58.1 53.1 920-923 bp 1.0 41.0
ndhF-rpl32_p3 GGAAGTGGGAGAAGAGGGAT TTGACATTGAGGTACGTTTT 55.0 35.0 58.1 53.1 921-924 bp 1.0 41.0
ndhF-rpl32_p4 GGAAGTGGGAGAAGAGGGAT TTTGACATTGAGGTACGTTT 55.0 35.0 58.1 53.1 922-925 bp 1.0 41.0
ndhF-rpl32_p5 GGAAGTGGGAGAAGAGGGAT TTTTGACATTGAGGTACGTT 55.0 35.0 58.1 53.1 923-926 bp 1.0 41.0