Hotspot

petA-psbJ

LSC · 60696-61519 · 824 bp

Back to Schizachne

Pi 0.00486

MarkerSeek score 61.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 824
variable_sites: 4
pi: 0.00486
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GCATCCGTTATTTTGGCACA	TCCTCTTTGGCTGATAGGTACT	45.0	45.5	57.4	58.0	1008-1009 bp	1.0	59.2
petA-psbJ_p2	TGCATCCGTTATTTTGGCAC	TCCTCTTTGGCTGATAGGTACT	45.0	45.5	57.4	58.0	1009-1010 bp	1.0	59.2
petA-psbJ_p3	CGTCCAAGGCCTTTTGTTCT	TCCTCTTTGGCTGATAGGTACT	50.0	45.5	58.7	58.0	1033-1034 bp	1.0	58.9
petA-psbJ_p4	GCATCCGTTATTTTGGCACA	CCTCTTTGGCTGATAGGTACTG	45.0	50.0	57.4	57.9	1007-1008 bp	1.0	58.7
petA-psbJ_p5	TGCATCCGTTATTTTGGCAC	CCTCTTTGGCTGATAGGTACTG	45.0	50.0	57.4	57.9	1008-1009 bp	1.0	58.7