Hotspot

rbcL-psaI

LSC · 56648-57607 · 960 bp

Back to Schizachne

Pi 0.00417

MarkerSeek score 61.10

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 960
variable_sites: 4
pi: 0.00417
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	TCGAGTTCGAGCCAGTAGAT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1065-1066 bp	1.0	79.8
rbcL-psaI_p2	TCGAGTTCGAGCCAGTAGAT	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.0	57.9	1071-1072 bp	1.0	79.2
rbcL-psaI_p3	AATGGAGTCCTGAACTTGCC	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.8	58.0	1116-1117 bp	1.0	78.4
rbcL-psaI_p4	CGCAGCTTGTGAAGTATGGA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.3	58.0	1097-1098 bp	1.0	77.9
rbcL-psaI_p5	AATGGAGTCCTGAACTTGCC	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.8	57.9	1122-1123 bp	1.0	77.8