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psbM-petN

LSC · 17398-18163 · 766 bp

Back to Schizachne

Pi 0.00814

MarkerSeek score 61.40

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 766
variable_sites: 6
pi: 0.00814
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TGCTACTGCACTGTTCATTCT	TAGTAAGTCTCGCTTGGGCT	42.9	50.0	57.6	58.2	906-928 bp	1.0	70.6
psbM-petN_p2	TGCTACTGCACTGTTCATTCT	GTAGTATGGGGGAGGAGTGG	42.9	60.0	57.6	58.3	850-872 bp	1.0	69.7
psbM-petN_p3	TGCTACTGCACTGTTCATTCT	TAGTATGGGGGAGGAGTGGA	42.9	55.0	57.6	58.4	849-871 bp	1.0	69.0
psbM-petN_p4	TGCTACTGCACTGTTCATTC	TAGTAAGTCTCGCTTGGGCT	45.0	50.0	56.1	58.2	906-928 bp	1.0	66.4
psbM-petN_p5	TGCTACTGCACTGTTCATTCT	AGTAGTATGGGGGAGGAGTG	42.9	55.0	57.6	57.2	851-873 bp	1.0	65.8