Hotspot

psbK-psbI

LSC · 7224-7622 · 399 bp

Back to Schizachne

Pi 0.0251

MarkerSeek score 69.00

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 399
variable_sites: 10
pi: 0.0251
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CTTTTGTTTGGCAAGCTGCT	ACGTCCTGGGTCATTGGATA	45.0	50.0	58.1	58.1	524 bp	1.0	80.1
psbK-psbI_p2	CTTTTGTTTGGCAAGCTGCT	TACGTCCTGGGTCATTGGAT	45.0	50.0	58.1	58.1	525 bp	1.0	80.1
psbK-psbI_p3	GTTTGGCAAGCTGCTGTAAG	ACGTCCTGGGTCATTGGATA	50.0	50.0	57.9	58.1	519 bp	1.0	80.1
psbK-psbI_p4	GTTTGGCAAGCTGCTGTAAG	TACGTCCTGGGTCATTGGAT	50.0	50.0	57.9	58.1	520 bp	1.0	80.1
psbK-psbI_p5	CTTTTGTTTGGCAAGCTGCT	TTTTTACTCCTCACGCCCAG	45.0	50.0	58.1	57.8	548 bp	1.0	79.6