Hotspot

trnL-TAG-ccsA

SSC · 116311-116413 · 103 bp

Back to Schisandra

Pi 0.0537
MarkerSeek score 66.50
Primer available yes
Label trnL-TAG-ccsA

Feature Metadata

label_name
trnL-TAG-ccsA
feature_type
igs
region
SSC
length_bp
103
variable_sites
11
pi
0.0537
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-TAG-ccsA_p1 TAGACACGCTGCTCTTAGGA TTTCAGTTGAAAGCCCCGAA 50.0 45.0 57.9 57.9 523-528 bp 1.0 82.8
trnL-TAG-ccsA_p2 AAATTGGTAGACACGCTGCT TTTCAGTTGAAAGCCCCGAA 45.0 45.0 57.8 57.9 530-535 bp 1.0 82.3
trnL-TAG-ccsA_p3 GTAGACACGCTGCTCTTAGG TTTCAGTTGAAAGCCCCGAA 55.0 45.0 57.8 57.9 524-529 bp 1.0 82.2
trnL-TAG-ccsA_p4 TAGACACGCTGCTCTTAGGA CTCACCAAACGAAAATGGCC 50.0 50.0 57.9 57.9 723-728 bp 1.0 82.1
trnL-TAG-ccsA_p5 AAATTGGTAGACACGCTGCT CTCACCAAACGAAAATGGCC 45.0 50.0 57.8 57.9 730-735 bp 1.0 81.6