Hotspot

ndhF-rpl32

SSC · 114364-115373 · 1010 bp

Back to Schisandra

Pi 0.0419

MarkerSeek score 79.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1010
variable_sites: 83
pi: 0.0419
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AACACATTCCCACCAGTTCC	GCTTTTTCTGCGGTCCAATC	50.0	50.0	58.0	58.0	1536-1610 bp	1.0	88.5
ndhF-rpl32_p2	CATTCCCACCAGTTCCCAAA	GCTTTTTCTGCGGTCCAATC	50.0	50.0	58.0	58.0	1532-1606 bp	1.0	88.5
ndhF-rpl32_p3	AACACATTCCCACCAGTTCC	AATCCCCCTTTCTCCTCCAA	50.0	50.0	58.0	57.9	1520-1594 bp	1.0	88.1
ndhF-rpl32_p4	AACACATTCCCACCAGTTCC	ATCCCCCTTTCTCCTCCAAA	50.0	50.0	58.0	57.9	1519-1593 bp	1.0	88.1
ndhF-rpl32_p5	AACACATTCCCACCAGTTCC	TCCCCCTTTCTCCTCCAAAT	50.0	50.0	58.0	57.9	1518-1592 bp	1.0	88.1