Hotspot

psaA-ycf3

LSC · 43973-44605 · 633 bp

Back to Schisandra

Pi 0.0184

MarkerSeek score 65.30

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 633
variable_sites: 23
pi: 0.0184
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	TTGGTTGAAGATCACGAGGC	50.0	50.0	57.9	58.2	744-754 bp	1.0	80.0
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	GCTATAGCGCTTACTCCAGG	50.0	55.0	57.9	57.6	785-795 bp	1.0	78.6
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	CCGATCAAGCTGCTGAGTAT	50.0	50.0	57.9	57.5	814-824 bp	1.0	77.1
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	TCCGATCAAGCTGCTGAGTA	50.0	50.0	57.9	58.5	815-825 bp	1.0	77.1
psaA-ycf3_p5	TGTTCTTGAGAAATGGCCGG	TTGGTTGAAGATCACGAGGC	50.0	50.0	58.5	58.2	769-779 bp	1.0	77.1