Hotspot

trnE-TTC-trnT-GGT

LSC · 33175-33544 · 370 bp

Back to Schisandra

Pi 0.0319

MarkerSeek score 70.70

Primer available yes

Label trnE-TTC-trnT-GGT

Feature Metadata

label_name: trnE-TTC-trnT-GGT
feature_type: igs
region: LSC
length_bp: 370
variable_sites: 25
pi: 0.0319
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-TTC-trnT-GGT_p1	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	451-453 bp	1.0	83.0
trnE-TTC-trnT-GGT_p2	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	472-474 bp	1.0	82.9
trnE-TTC-trnT-GGT_p3	TTCCTAGTACCCTACCCCCA	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	509-511 bp	1.0	82.3
trnE-TTC-trnT-GGT_p4	TGTCCTGAACCACTAGACGA	GACTAAGACTGGCGTTTCGT	50.0	50.0	57.7	57.9	495-497 bp	1.0	82.1
trnE-TTC-trnT-GGT_p5	GCTGCCTCCTTGAAAGAGAG	GACTAAGACTGGCGTTTCGT	55.0	50.0	58.3	57.9	516-518 bp	1.0	82.0