Hotspot

psbM-trnD-GTC

LSC · 31494-32470 · 977 bp

Back to Schisandra

Pi 0.0229

MarkerSeek score 67.00

Primer available yes

Label psbM-trnD-GTC

Feature Metadata

label_name: psbM-trnD-GTC
feature_type: igs
region: LSC
length_bp: 977
variable_sites: 42
pi: 0.0229
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GTC_p1	AGAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.8	58.5	749-1183 bp	1.0	65.7
psbM-trnD-GTC_p2	AGGAACTAGAATGAACAGTGCA	TTCAATTGGTCAGAGCACCG	40.9	50.0	57.4	58.5	756-1190 bp	1.0	62.6
psbM-trnD-GTC_p3	AGAATGAACAGTGCAGTAGC	GTTCAATTGGTCAGAGCACC	45.0	50.0	55.8	57.0	750-1184 bp	1.0	61.6
psbM-trnD-GTC_p4	AGAATGAACAGTGCAGTAGC	CCCCGGGATTGTAGTTCAATT	45.0	47.6	55.8	58.0	763-1197 bp	1.0	61.4
psbM-trnD-GTC_p5	GGAACTAGAATGAACAGTGCAG	TTCAATTGGTCAGAGCACCG	45.5	50.0	57.1	58.5	755-1189 bp	1.0	60.1