Hotspot

atpH-atpI

LSC · 15209-16325 · 1117 bp

Back to Schisandra

Pi 0.041

MarkerSeek score 69.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1117
variable_sites: 88
pi: 0.041
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	CAAACAATTCCAACCGACGG	45.0	50.0	58.0	58.0	1471-1728 bp	1.0	88.2
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	TATGGTCCTTGGGTTCCCTT	45.0	50.0	58.0	57.9	1384-1641 bp	1.0	88.1
atpH-atpI_p3	AGCAAGGTCAAGGCAATCAA	CAAACAATTCCAACCGACGG	45.0	50.0	58.0	58.0	1746-2004 bp	1.0	88.0
atpH-atpI_p4	AGCAAGGTCAAGGCAATCAA	TATGGTCCTTGGGTTCCCTT	45.0	50.0	58.0	57.9	1659-1917 bp	1.0	87.9
atpH-atpI_p5	TAGGTCCCAGGTATCGTGTC	CAAACAATTCCAACCGACGG	55.0	50.0	57.9	58.0	2157-2212 bp	0.666667	70.7