Hotspot

psbI-trnS-GCT

LSC · 9252-9406 · 155 bp

Back to Schisandra

Pi 0.0773

MarkerSeek score 71.40

Primer available yes

Label psbI-trnS-GCT

Feature Metadata

label_name: psbI-trnS-GCT
feature_type: igs
region: LSC
length_bp: 155
variable_sites: 23
pi: 0.0773
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCT_p1	CTCCATCAAGCAAAGACGGT	TGGACTAAAGCGTCGGATTG	50.0	50.0	58.2	58.0	549-567 bp	1.0	87.6
psbI-trnS-GCT_p2	CTCCATCAAGCAAAGACGGT	GTGGACTAAAGCGTCGGATT	50.0	50.0	58.2	58.0	550-568 bp	1.0	87.6
psbI-trnS-GCT_p3	TATTCCGGTCCCCCACAATA	TGGACTAAAGCGTCGGATTG	50.0	50.0	57.8	58.0	530-547 bp	0.833333	79.9
psbI-trnS-GCT_p4	TTTTCTTCGCCAAATTGCCC	TGGACTAAAGCGTCGGATTG	45.0	50.0	57.8	58.0	857-860 bp	0.666667	67.6
psbI-trnS-GCT_p5	TTTTCTTCGCCAAATTGCCC	GTGGACTAAAGCGTCGGATT	45.0	50.0	57.8	58.0	858-861 bp	0.666667	67.6