Hotspot

rps16-trnQ-TTG

LSC · 6377-8162 · 1786 bp

Back to Schisandra

Pi 0.0205

MarkerSeek score 63.90

Primer available yes

Label rps16-trnQ-TTG

Feature Metadata

label_name: rps16-trnQ-TTG
feature_type: igs
region: LSC
length_bp: 1786
variable_sites: 75
pi: 0.0205
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-TTG_p1	ATCATGTCGTTCAAGTCGCA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.9	58.1	1520-2037 bp	1.0	86.1
rps16-trnQ-TTG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1508-2025 bp	1.0	85.6
rps16-trnQ-TTG_p3	ATCATGTCGTTCAAGTCGCA	CTCGGAGGTTCGAATCCTTC	45.0	55.0	57.9	57.8	1524-2041 bp	1.0	85.0
rps16-trnQ-TTG_p4	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	45.0	55.0	58.1	57.8	1512-2029 bp	1.0	84.5
rps16-trnQ-TTG_p5	ATCATGTCGTTCAAGTCGCA	TACTCGGAGGTTCGAATCCT	45.0	50.0	57.9	57.6	1526-2043 bp	1.0	83.2