Hotspot

matK-rps16

LSC · 3752-5296 · 1545 bp

Back to Schisandra

Pi 0.0198

MarkerSeek score 54.70

Primer available yes

Label matK-rps16

Feature Metadata

label_name: matK-rps16
feature_type: igs
region: LSC
length_bp: 1545
variable_sites: 64
pi: 0.0198
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	AGGAAACGTTGTTGGCAAGA	GGAACTTCGAGTTAATCAAACGA	45.0	39.1	58.2	57.2	1629-1661 bp	1.0	50.9
matK-rps16_p2	AGGAAACGTTGTTGGCAAGA	AGGAACTTCGAGTTAATCAAACG	45.0	39.1	58.2	57.2	1630-1662 bp	1.0	50.9
matK-rps16_p3	AAGTGCGTAGATCGACTCCT	GGAACTTCGAGTTAATCAAACGA	50.0	39.1	58.2	57.2	1666-1698 bp	1.0	50.8
matK-rps16_p4	TGCGTAGATCGACTCCTGAA	GGAACTTCGAGTTAATCAAACGA	50.0	39.1	58.3	57.2	1663-1695 bp	1.0	50.8
matK-rps16_p5	AAGTGCGTAGATCGACTCCT	AGGAACTTCGAGTTAATCAAACG	50.0	39.1	58.2	57.2	1667-1699 bp	1.0	50.8