primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	matK-rps16	1	AGGAAACGTTGTTGGCAAGA	GGAACTTCGAGTTAATCAAACGA	20	23	45.0	39.1	58.2	57.2	0.0	0.0	4.076557	3752	5296	1629-1661	1.000000	50.9
matK-rps16_p2	matK-rps16	2	AGGAAACGTTGTTGGCAAGA	AGGAACTTCGAGTTAATCAAACG	20	23	45.0	39.1	58.2	57.2	0.0	1.1	4.083257	3752	5296	1630-1662	1.000000	50.9
matK-rps16_p3	matK-rps16	3	AAGTGCGTAGATCGACTCCT	GGAACTTCGAGTTAATCAAACGA	20	23	50.0	39.1	58.2	57.2	4.4	0.0	4.081556	3752	5296	1666-1698	1.000000	50.8
matK-rps16_p4	matK-rps16	4	TGCGTAGATCGACTCCTGAA	GGAACTTCGAGTTAATCAAACGA	20	23	50.0	39.1	58.3	57.2	4.4	0.0	4.087769	3752	5296	1663-1695	1.000000	50.8
matK-rps16_p5	matK-rps16	5	AAGTGCGTAGATCGACTCCT	AGGAACTTCGAGTTAATCAAACG	20	23	50.0	39.1	58.2	57.2	4.4	1.1	4.088257	3752	5296	1667-1699	1.000000	50.8
rps16-trnQ-TTG_p1	rps16-trnQ-TTG	1	ATCATGTCGTTCAAGTCGCA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	57.9	58.1	0.0	16.9	0.154950	6377	8162	1520-2037	1.000000	86.1
rps16-trnQ-TTG_p2	rps16-trnQ-TTG	2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6377	8162	1508-2025	1.000000	85.6
rps16-trnQ-TTG_p3	rps16-trnQ-TTG	3	ATCATGTCGTTCAAGTCGCA	CTCGGAGGTTCGAATCCTTC	20	20	45.0	55.0	57.9	57.8	0.0	16.9	0.290201	6377	8162	1524-2041	1.000000	85.0
rps16-trnQ-TTG_p4	rps16-trnQ-TTG	4	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	20	20	45.0	55.0	58.1	57.8	8.1	16.9	0.350677	6377	8162	1512-2029	1.000000	84.5
rps16-trnQ-TTG_p5	rps16-trnQ-TTG	5	ATCATGTCGTTCAAGTCGCA	TACTCGGAGGTTCGAATCCT	20	20	45.0	50.0	57.9	57.6	0.0	16.1	0.510802	6377	8162	1526-2043	1.000000	83.2
psbI-trnS-GCT_p1	psbI-trnS-GCT	1	CTCCATCAAGCAAAGACGGT	TGGACTAAAGCGTCGGATTG	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.191069	9252	9406	549-567	1.000000	87.6
psbI-trnS-GCT_p2	psbI-trnS-GCT	2	CTCCATCAAGCAAAGACGGT	GTGGACTAAAGCGTCGGATT	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.192457	9252	9406	550-568	1.000000	87.6
psbI-trnS-GCT_p3	psbI-trnS-GCT	3	TATTCCGGTCCCCCACAATA	TGGACTAAAGCGTCGGATTG	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.198149	9252	9406	530-547	0.833333	79.9
psbI-trnS-GCT_p4	psbI-trnS-GCT	4	TTTTCTTCGCCAAATTGCCC	TGGACTAAAGCGTCGGATTG	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.166902	9252	9406	857-860	0.666667	67.6
psbI-trnS-GCT_p5	psbI-trnS-GCT	5	TTTTCTTCGCCAAATTGCCC	GTGGACTAAAGCGTCGGATT	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.168290	9252	9406	858-861	0.666667	67.6
trnS-GCT-trnR-TCT_p1	trnS-GCT-trnR-TCT	1	CAATCCGACGCTTTAGTCCA	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.056187	9496	11386	1417-1977	1.000000	71.8
trnS-GCT-trnR-TCT_p2	trnS-GCT-trnR-TCT	2	AATCCGACGCTTTAGTCCAC	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.057575	9496	11386	1416-1976	1.000000	71.7
trnS-GCT-trnR-TCT_p3	trnS-GCT-trnR-TCT	3	CAATCCGACGCTTTAGTCCA	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.139515	9496	11386	1423-1983	1.000000	71.1
trnS-GCT-trnR-TCT_p4	trnS-GCT-trnR-TCT	4	AATCCGACGCTTTAGTCCAC	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.140903	9496	11386	1422-1982	1.000000	71.1
trnS-GCT-trnR-TCT_p5	trnS-GCT-trnR-TCT	5	CGGAGAGAGAGGGATTCGAA	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	58.3	56.9	0.0	0.0	2.370066	9496	11386	1470-2030	1.000000	69.1
atpF-atpH_p1	atpF-atpH	1	GAAAGAATCGGTTACATTTTTCA	GCTTTTATTTGCGAATCCTTTTGT	23	24	30.4	33.3	53.9	57.5	0.0	1.3	11.562372	14461	14962	538-576	1.000000	51.7
atpF-atpH_p2	atpF-atpH	2	AAAGAATCGGTTACATTTTTCA	GCTTTTATTTGCGAATCCTTTTGT	22	24	27.3	33.3	52.6	57.5	0.0	1.3	11.875256	14461	14962	537-575	1.000000	51.7
atpF-atpH_p3	atpF-atpH	3	GAAAGAATCGGTTACATTTTTCAT	GCTTTTATTTGCGAATCCTTTTGT	24	24	29.2	33.3	54.5	57.5	0.0	1.3	12.005481	14461	14962	538-576	1.000000	51.7
atpF-atpH_p4	atpF-atpH	4	AAGAATCGGTTACATTTTTCAT	GCTTTTATTTGCGAATCCTTTTGT	22	24	27.3	33.3	52.4	57.5	0.0	1.3	12.100454	14461	14962	536-574	1.000000	51.7
atpF-atpH_p5	atpF-atpH	5	GAAAGAATCGGTTACATTTTTCA	GCTTTTATTTGCGAATCCTTTTG	23	23	30.4	34.8	53.9	56.0	0.0	1.3	12.126980	14461	14962	538-576	1.000000	51.7
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	CAAACAATTCCAACCGACGG	20	20	45.0	50.0	58.0	58.0	1.4	13.6	0.083275	15209	16325	1471-1728	1.000000	88.2
atpH-atpI_p2	atpH-atpI	2	ATAACGGAAGCGGCAGAAAT	TATGGTCCTTGGGTTCCCTT	20	20	45.0	50.0	58.0	57.9	1.4	9.4	0.107218	15209	16325	1384-1641	1.000000	88.1
atpH-atpI_p3	atpH-atpI	3	AGCAAGGTCAAGGCAATCAA	CAAACAATTCCAACCGACGG	20	20	45.0	50.0	58.0	58.0	0.0	13.6	0.045240	15209	16325	1746-2004	1.000000	88.0
atpH-atpI_p4	atpH-atpI	4	AGCAAGGTCAAGGCAATCAA	TATGGTCCTTGGGTTCCCTT	20	20	45.0	50.0	58.0	57.9	0.0	9.4	0.069183	15209	16325	1659-1917	1.000000	87.9
atpH-atpI_p5	atpH-atpI	5	TAGGTCCCAGGTATCGTGTC	CAAACAATTCCAACCGACGG	20	20	55.0	50.0	57.9	58.0	0.0	13.6	0.107858	15209	16325	2157-2212	0.666667	70.7
psbM-trnD-GTC_p1	psbM-trnD-GTC	1	AGAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	20	20	45.0	50.0	55.8	58.5	0.0	5.4	2.706630	31494	32470	749-1183	1.000000	65.7
psbM-trnD-GTC_p2	psbM-trnD-GTC	2	AGGAACTAGAATGAACAGTGCA	TTCAATTGGTCAGAGCACCG	22	20	40.9	50.0	57.4	58.5	0.0	5.4	3.101264	31494	32470	756-1190	1.000000	62.6
psbM-trnD-GTC_p3	psbM-trnD-GTC	3	AGAATGAACAGTGCAGTAGC	GTTCAATTGGTCAGAGCACC	20	20	45.0	50.0	55.8	57.0	0.0	5.4	3.226784	31494	32470	750-1184	1.000000	61.6
psbM-trnD-GTC_p4	psbM-trnD-GTC	4	AGAATGAACAGTGCAGTAGC	CCCCGGGATTGTAGTTCAATT	20	21	45.0	47.6	55.8	58.0	0.0	26.3	3.242233	31494	32470	763-1197	1.000000	61.4
psbM-trnD-GTC_p5	psbM-trnD-GTC	5	GGAACTAGAATGAACAGTGCAG	TTCAATTGGTCAGAGCACCG	22	20	45.5	50.0	57.1	58.5	0.0	5.4	3.413216	31494	32470	755-1189	1.000000	60.1
trnD-GTC-trnY-GTA_p1	trnD-GTC-trnY-GTA	1	CGGTGCTCTGACCAATTGAA	CTGGTTCAAATCCAGCTCGA	20	20	50.0	50.0	58.5	57.9	7.1	0.0	0.576473	32546	32957	463-475	1.000000	84.5
trnD-GTC-trnY-GTA_p2	trnD-GTC-trnY-GTA	2	CGGTGCTCTGACCAATTGAA	TGGTTCAAATCCAGCTCGAC	20	20	50.0	50.0	58.5	58.2	7.1	0.0	0.668921	32546	32957	462-474	1.000000	83.7
trnD-GTC-trnY-GTA_p3	trnD-GTC-trnY-GTA	3	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	20	20	50.0	55.0	58.5	58.4	7.1	0.0	0.828828	32546	32957	500-512	1.000000	81.8
trnD-GTC-trnY-GTA_p4	trnD-GTC-trnY-GTA	4	CGGTGCTCTGACCAATTGAA	GGGACGGACTGTAAATTCGT	20	20	50.0	50.0	58.5	57.6	7.1	0.0	0.852505	32546	32957	499-511	1.000000	81.6
trnD-GTC-trnY-GTA_p5	trnD-GTC-trnY-GTA	5	CGGTGCTCTGACCAATTGAA	GGACTGTAAATTCGTTGGCG	20	20	50.0	50.0	58.5	57.5	7.1	0.0	0.998018	32546	32957	494-506	1.000000	80.5
trnE-TTC-trnT-GGT_p1	trnE-TTC-trnT-GGT	1	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	33175	33544	451-453	1.000000	83.0
trnE-TTC-trnT-GGT_p2	trnE-TTC-trnT-GGT	2	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	33175	33544	472-474	1.000000	82.9
trnE-TTC-trnT-GGT_p3	trnE-TTC-trnT-GGT	3	TTCCTAGTACCCTACCCCCA	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.295576	33175	33544	509-511	1.000000	82.3
trnE-TTC-trnT-GGT_p4	trnE-TTC-trnT-GGT	4	TGTCCTGAACCACTAGACGA	GACTAAGACTGGCGTTTCGT	20	20	50.0	50.0	57.7	57.9	0.0	0.0	0.343279	33175	33544	495-497	1.000000	82.1
trnE-TTC-trnT-GGT_p5	trnE-TTC-trnT-GGT	5	GCTGCCTCCTTGAAAGAGAG	GACTAAGACTGGCGTTTCGT	20	20	55.0	50.0	58.3	57.9	0.0	0.0	0.325128	33175	33544	516-518	1.000000	82.0
psaA-ycf3_p1	psaA-ycf3	1	GCCCATTCCTCGAAAGAAGT	TTGGTTGAAGATCACGAGGC	20	20	50.0	50.0	57.9	58.2	4.1	0.0	0.306932	43973	44605	744-754	1.000000	80.0
psaA-ycf3_p2	psaA-ycf3	2	GCCCATTCCTCGAAAGAAGT	GCTATAGCGCTTACTCCAGG	20	20	50.0	55.0	57.9	57.6	4.1	18.7	0.471060	43973	44605	785-795	1.000000	78.6
psaA-ycf3_p3	psaA-ycf3	3	GCCCATTCCTCGAAAGAAGT	CCGATCAAGCTGCTGAGTAT	20	20	50.0	50.0	57.9	57.5	4.1	1.4	0.638178	43973	44605	814-824	1.000000	77.1
psaA-ycf3_p4	psaA-ycf3	4	GCCCATTCCTCGAAAGAAGT	TCCGATCAAGCTGCTGAGTA	20	20	50.0	50.0	57.9	58.5	4.1	1.4	0.641013	43973	44605	815-825	1.000000	77.1
psaA-ycf3_p5	psaA-ycf3	5	TGTTCTTGAGAAATGGCCGG	TTGGTTGAAGATCACGAGGC	20	20	50.0	50.0	58.5	58.2	0.0	0.0	0.657964	43973	44605	769-779	1.000000	77.1
trnT-TGT-trnF-GAA_p1	trnT-TGT-trnF-GAA	1	GCGATGCTCTAACCTCTGAG	TCTACCAGCTGAGCTATCCC	20	20	55.0	55.0	57.9	58.0	0.0	17.4	0.135246	48756	50274	1422-1573	1.000000	85.8
trnT-TGT-trnF-GAA_p2	trnT-TGT-trnF-GAA	2	GCGATGCTCTAACCTCTGAG	ATTATCCACGTGCCAGGAAC	20	20	55.0	50.0	57.9	58.0	0.0	10.9	0.171127	48756	50274	1482-1633	1.000000	85.4
trnT-TGT-trnF-GAA_p3	trnT-TGT-trnF-GAA	3	GCGATGCTCTAACCTCTGAG	TTTGAACTGGTGACACGAGG	20	20	55.0	50.0	57.9	58.1	0.0	20.1	0.184636	48756	50274	1458-1609	1.000000	85.4
trnT-TGT-trnF-GAA_p4	trnT-TGT-trnF-GAA	4	GCGATGCTCTAACCTCTGAG	GTGCCAGGAACCAGATTTGA	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.223040	48756	50274	1473-1624	1.000000	85.0
trnT-TGT-trnF-GAA_p5	trnT-TGT-trnF-GAA	5	GGAATCGAACCGATGACCAT	TCTACCAGCTGAGCTATCCC	20	20	50.0	55.0	57.8	58.0	15.5	17.4	0.242999	48756	50274	1454-1605	1.000000	84.9
petB-petD_p1	petB-petD	1	CAATCCACTTTGACCCGTTT	CTTAGAGATCCATCCCCCGA	20	20	45.0	55.0	56.5	57.7	0.0	0.0	1.775848	78480	79332	960-1006	1.000000	70.5
petB-petD_p2	petB-petD	2	CAATCCACTTTGACCCGTTT	TAGAGATCCATCCCCCGAAG	20	20	45.0	55.0	56.5	57.7	0.0	0.0	1.775848	78480	79332	958-1004	1.000000	70.5
petB-petD_p3	petB-petD	3	CAATCCACTTTGACCCGTTT	TCTTAGAGATCCATCCCCCG	20	20	45.0	55.0	56.5	57.7	0.0	0.0	1.775848	78480	79332	961-1007	1.000000	70.5
petB-petD_p4	petB-petD	4	CAATCCACTTTGACCCGTTT	CCCATCCCTTTAGCCGATTT	20	20	45.0	50.0	56.5	57.6	0.0	0.0	1.830041	78480	79332	1037-1083	1.000000	69.9
petB-petD_p5	petB-petD	5	CAATCCACTTTGACCCGTTTT	TAGAGATCCATCCCCCGAAG	21	20	42.9	55.0	57.3	57.7	0.0	0.0	2.037070	78480	79332	958-1004	1.000000	68.4
rps8-rpl14_p1	rps8-rpl14	1	GGCAATAGTGTCCCTACCCA	GAACTCGAGTTTTTGGTGCG	20	20	55.0	50.0	58.8	58.0	0.0	30.2	0.809096	82659	82830	273-384	1.000000	77.4
rps8-rpl14_p2	rps8-rpl14	2	TGCTATTCGAACCGTTCCTT	GAACTCGAGTTTTTGGTGCG	20	20	45.0	50.0	57.2	58.0	0.0	30.2	0.773663	82659	82830	333-444	1.000000	77.1
rps8-rpl14_p3	rps8-rpl14	3	TCGGCAATAGTGTCCCTACC	GAACTCGAGTTTTTGGTGCG	20	20	55.0	50.0	58.9	58.0	0.0	30.2	0.901567	82659	82830	275-386	1.000000	76.7
rps8-rpl14_p4	rps8-rpl14	4	GGCAATAGTGTCCCTACCCA	ACTCGAGTTTTTGGTGCGAT	20	20	55.0	45.0	58.8	58.1	0.0	13.6	0.911426	82659	82830	271-382	1.000000	76.6
rps8-rpl14_p5	rps8-rpl14	5	TGCTATTCGAACCGTTCCTT	ACTCGAGTTTTTGGTGCGAT	20	20	45.0	45.0	57.2	58.1	0.0	13.6	0.875993	82659	82830	331-442	1.000000	76.3
ycf2_p1	ycf2	1	GAACCAAGGAATCCGTTCGA	AACCAACAACTCGAAGTGCT	20	20	50.0	45.0	57.9	58.0	3.4	0.0	0.127336	94831	94950	591-693	1.000000	90.1
ycf2_p2	ycf2	2	GAACCAAGGAATCCGTTCGA	ACCAACAACTCGAAGTGCTT	20	20	50.0	45.0	57.9	58.0	3.4	0.0	0.127336	94831	94950	590-692	1.000000	90.1
ycf2_p3	ycf2	3	TCGGTCGCTCAAGATCTTTG	AACCAACAACTCGAAGTGCT	20	20	50.0	45.0	58.0	58.0	4.9	0.0	0.042095	94831	94950	780-888	1.000000	89.8
ycf2_p4	ycf2	4	TCGGTCGCTCAAGATCTTTG	ACCAACAACTCGAAGTGCTT	20	20	50.0	45.0	58.0	58.0	4.9	0.0	0.042095	94831	94950	779-887	1.000000	89.8
ycf2_p5	ycf2	5	GGTCGCTCAAGATCTTTGGT	ACCAACAACTCGAAGTGCTT	20	20	50.0	45.0	57.9	58.0	4.9	0.0	0.138992	94831	94950	777-885	1.000000	89.1
ndhF-rpl32_p1	ndhF-rpl32	1	AACACATTCCCACCAGTTCC	GCTTTTTCTGCGGTCCAATC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.022012	114364	115373	1536-1610	1.000000	88.5
ndhF-rpl32_p2	ndhF-rpl32	2	CATTCCCACCAGTTCCCAAA	GCTTTTTCTGCGGTCCAATC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.025630	114364	115373	1532-1606	1.000000	88.5
ndhF-rpl32_p3	ndhF-rpl32	3	AACACATTCCCACCAGTTCC	AATCCCCCTTTCTCCTCCAA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.079546	114364	115373	1520-1594	1.000000	88.1
ndhF-rpl32_p4	ndhF-rpl32	4	AACACATTCCCACCAGTTCC	ATCCCCCTTTCTCCTCCAAA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.079546	114364	115373	1519-1593	1.000000	88.1
ndhF-rpl32_p5	ndhF-rpl32	5	AACACATTCCCACCAGTTCC	TCCCCCTTTCTCCTCCAAAT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.079546	114364	115373	1518-1592	1.000000	88.1
trnL-TAG-ccsA_p1	trnL-TAG-ccsA	1	TAGACACGCTGCTCTTAGGA	TTTCAGTTGAAAGCCCCGAA	20	20	50.0	45.0	57.9	57.9	0.0	3.3	0.184327	116311	116413	523-528	1.000000	82.8
trnL-TAG-ccsA_p2	trnL-TAG-ccsA	2	AAATTGGTAGACACGCTGCT	TTTCAGTTGAAAGCCCCGAA	20	20	45.0	45.0	57.8	57.9	0.0	3.3	0.245054	116311	116413	530-535	1.000000	82.3
trnL-TAG-ccsA_p3	trnL-TAG-ccsA	3	GTAGACACGCTGCTCTTAGG	TTTCAGTTGAAAGCCCCGAA	20	20	55.0	45.0	57.8	57.9	0.0	3.3	0.256345	116311	116413	524-529	1.000000	82.2
trnL-TAG-ccsA_p4	trnL-TAG-ccsA	4	TAGACACGCTGCTCTTAGGA	CTCACCAAACGAAAATGGCC	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.188850	116311	116413	723-728	1.000000	82.1
trnL-TAG-ccsA_p5	trnL-TAG-ccsA	5	AAATTGGTAGACACGCTGCT	CTCACCAAACGAAAATGGCC	20	20	45.0	50.0	57.8	57.9	0.0	0.0	0.249577	116311	116413	730-735	1.000000	81.6
rps15-ycf1_p1	rps15-ycf1	1	CCGCAGACCTCTCTGAGATA	GAACGACATGGAAGGATGCT	20	20	55.0	50.0	58.0	58.0	8.0	0.0	0.055573	125625	126007	894-946	1.000000	86.4
rps15-ycf1_p2	rps15-ycf1	2	TTTGGCTCGCGAATATCCAA	GAACGACATGGAAGGATGCT	20	20	45.0	50.0	58.0	58.0	20.6	0.0	0.061097	125625	126007	995-1047	1.000000	86.2
rps15-ycf1_p3	rps15-ycf1	3	AACACCATGCATTGATGGGT	GAACGACATGGAAGGATGCT	20	20	45.0	50.0	58.1	58.0	8.9	0.0	0.088461	125625	126007	1184-1236	1.000000	85.5
rps15-ycf1_p4	rps15-ycf1	4	TACGACTCAGCTCCAGCATA	GAACGACATGGAAGGATGCT	20	20	50.0	50.0	57.9	58.0	0.5	0.0	0.082621	125625	126007	1440-1492	1.000000	84.6
rps15-ycf1_p5	rps15-ycf1	5	TATACGACTCAGCTCCAGCA	GAACGACATGGAAGGATGCT	20	20	50.0	50.0	57.9	58.0	0.5	0.0	0.082621	125625	126007	1442-1494	1.000000	84.5