Hotspot

ycf1

SSC · 127016-132667 · 5652 bp

Pi 0.00236

MarkerSeek score 41.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5652
variable_sites: 51
pi: 0.00236
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGAATCCATTCTTCCTTGTTC	ACGGATACTGATTACCGAGA	38.1	45.0	53.8	55.2	168 bp	1.0	42.4
ycf1_p2	TTGAATCCATTCTTCCTTGTTC	ACGGATACTGATTACCGAGA	36.4	45.0	54.6	55.2	169 bp	1.0	42.4
ycf1_p3	TGAATCCATTCTTCCTTGTTC	ACGGATACTGATTACCGAGAA	38.1	42.9	53.8	56.0	168 bp	1.0	42.4
ycf1_p4	TTGAATCCATTCTTCCTTGTTC	ACGGATACTGATTACCGAGAA	36.4	42.9	54.6	56.0	169 bp	1.0	42.4
ycf1_p5	TTTGAATCCATTCTTCCTTGTTC	ACGGATACTGATTACCGAGA	34.8	45.0	55.4	55.2	170 bp	1.0	42.4