Hotspot

rps15

SSC · 126367-126639 · 273 bp

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Pi 0.00483
MarkerSeek score 25.70
Primer available yes
Label rps15

Feature Metadata

label_name
rps15
feature_type
gene
region
SSC
length_bp
273
variable_sites
3
pi
0.00483
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
25.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15_p1 AACACCATGCATTGATGGGT AGACTAGGCATGGTGTGGAT 45.0 50.0 58.1 58.1 1067 bp 1.0 79.7
rps15_p2 AGTCTGCGCACCAATATCTG AGACTAGGCATGGTGTGGAT 50.0 50.0 58.1 58.1 1379 bp 1.0 79.5
rps15_p3 TTCCCAATTGTTCTGGTGCA AGACTAGGCATGGTGTGGAT 45.0 50.0 57.9 58.1 1263 bp 1.0 79.4
rps15_p4 CTTGTAGCTGGTGCAGTCAT AGACTAGGCATGGTGTGGAT 50.0 50.0 57.9 58.1 1012 bp 1.0 79.4
rps15_p5 TAGCTGGTGCAGTCATAGGT AGACTAGGCATGGTGTGGAT 50.0 50.0 58.1 58.1 1008 bp 1.0 79.2