Hotspot

rpl16

LSC · 84233-85639 · 1407 bp

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Pi 0.00383
MarkerSeek score 40.20
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1407
variable_sites
17
pi
0.00383
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 CCCAAGACCTATTTCCTTTGGT TCAAAACAAAAGCGAGCTCTT 45.5 38.1 58.0 56.9 1509-1511 bp 1.0 48.2
rpl16_p2 CCCAAGACCTATTTCCTTTGGT TCAAAACAAAAGCGAGCTCT 45.5 40.0 58.0 56.2 1509-1511 bp 0.928571 47.3
rpl16_p3 TCCCAAGACCTATTTCCTTTGG TCAAAACAAAAGCGAGCTCT 45.5 40.0 57.7 56.2 1510-1512 bp 0.928571 45.1
rpl16_p4 CCCAAGACCTATTTCCTTTGGT ATCAAAACAAAAGCGAGCTCT 45.5 38.1 58.0 56.7 1510-1512 bp 0.928571 43.5
rpl16_p5 CCCAAGACCTATTTCCTTTGGT TCAATCAAAACAAAAGCGAGCT 45.5 36.4 58.0 57.7 1513-1515 bp 0.928571 43.3