Hotspot

petD

LSC · 79490-80734 · 1245 bp

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Pi 0.00162
MarkerSeek score 46.80
Primer available yes
Label petD

Feature Metadata

label_name
petD
feature_type
gene
region
LSC
length_bp
1245
variable_sites
7
pi
0.00162
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petD_p1 ATATTTCCCTTCAACCCCGC TCCCTAGATACACACGTCGT 50.0 50.0 57.9 57.6 1372 bp 1.0 76.6
petD_p2 TCCCTTCAACCCCGCTATAT TCCCTAGATACACACGTCGT 50.0 50.0 57.9 57.6 1367 bp 1.0 76.2
petD_p3 TTCCCTTCAACCCCGCTATA TCCCTAGATACACACGTCGT 50.0 50.0 58.1 57.6 1368 bp 1.0 76.2
petD_p4 ATATTTCCCTTCAACCCCGC ACTTGGAAGCGACTATTCCC 50.0 50.0 57.9 57.3 1388 bp 1.0 74.3
petD_p5 TCCCTTCAACCCCGCTATAT ACTTGGAAGCGACTATTCCC 50.0 50.0 57.9 57.3 1383 bp 1.0 74.0