Hotspot

ndhF

SSC · 113189-115435 · 2247 bp

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Pi 0.00235
MarkerSeek score 50.20
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2247
variable_sites
18
pi
0.00235
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 TGGGTTTCGACCAACAAACA AGATCTGGTGAATCGAAAACA 45.0 38.1 58.2 55.2 2334-2340 bp 0.928571 46.6
ndhF_p2 TGGGTTTCGACCAACAAACA AAGATCTGGTGAATCGAAAACA 45.0 36.4 58.2 56.0 2335-2341 bp 0.928571 44.8
ndhF_p3 TGGGTTTCGACCAACAAACA AGATCTGGTGAATCGAAAACAA 45.0 36.4 58.2 56.0 2334-2340 bp 0.928571 44.8
ndhF_p4 TGGGTTTCGACCAACAAACA AAGATCTGGTGAATCGAAAACAA 45.0 34.8 58.2 56.7 2335-2341 bp 0.928571 42.4
ndhF_p5 TGGGTTTCGACCAACAAACA GATCTGGTGAATCGAAAACA 45.0 40.0 58.2 53.6 2333-2339 bp 0.928571 41.8