Hotspot

rpl32-trnL-UAG

SSC · 116426-117333 · 908 bp

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Pi 0.00402
MarkerSeek score 45.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
908
variable_sites
14
pi
0.00402
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 ATTGGGCAGCCTTAAAAGCT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.1 57.9 1036-1054 bp 1.0 79.9
rpl32-trnL-UAG_p2 ATTGGGCAGCCTTAAAAGCT AGCAGCGTGTCTACCAATTT 45.0 45.0 58.1 57.8 1029-1047 bp 1.0 79.5
rpl32-trnL-UAG_p3 ATTGGGCAGCCTTAAAAGCT CCTAAGAGCAGCGTGTCTAC 45.0 55.0 58.1 57.8 1035-1053 bp 1.0 79.4
rpl32-trnL-UAG_p4 ATTGGGCAGCCTTAAAAGCT CTAAGAGCAGCGTGTCTACC 45.0 55.0 58.1 57.8 1034-1052 bp 1.0 79.4
rpl32-trnL-UAG_p5 ATTGGGCAGCCTTAAAAGCT GAGCAGCGTGTCTACCAATT 45.0 50.0 58.1 58.3 1030-1048 bp 1.0 78.7