Hotspot

petA-psbJ

LSC · 65604-66641 · 1038 bp

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Pi 0.0138
MarkerSeek score 60.40
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1038
variable_sites
34
pi
0.0138
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 CCATTACGTGTTCAAGGCCT ATTCCGGATTGGGTTCATCC 50.0 50.0 58.2 57.6 1178-1185 bp 1.0 78.4
petA-psbJ_p2 TCCATTACGTGTTCAAGGCC ATTCCGGATTGGGTTCATCC 50.0 50.0 58.2 57.6 1179-1186 bp 1.0 78.3
petA-psbJ_p3 GCATCTGTTGTTTTGGCACA ATTCCGGATTGGGTTCATCC 45.0 50.0 57.8 57.6 1145-1152 bp 1.0 78.1
petA-psbJ_p4 AGCATCTGTTGTTTTGGCAC ATTCCGGATTGGGTTCATCC 45.0 50.0 57.5 57.6 1146-1153 bp 1.0 75.7
petA-psbJ_p5 ACGTGTTCAAGGCCTTTTGT ATTCCGGATTGGGTTCATCC 45.0 50.0 58.5 57.6 1173-1180 bp 1.0 75.6