Hotspot

ndhC-trnV-UAC

LSC · 53341-53750 · 410 bp

Back to Schima

Pi 0.00236
MarkerSeek score 51.00
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
410
variable_sites
4
pi
0.00236
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGGGCTAAAACTCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 531-545 bp 1.0 81.6
ndhC-trnV-UAC_p2 CTGGTCCTTTGCTAATCGGG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.3 57.8 548-562 bp 1.0 79.9
ndhC-trnV-UAC_p3 CCTTTGCTAATCGGGGCTAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 543-557 bp 1.0 79.9
ndhC-trnV-UAC_p4 GGGGCTAAAACTCCGGAAAT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.9 57.5 530-544 bp 1.0 78.9
ndhC-trnV-UAC_p5 TCCTTTGCTAATCGGGGCTA AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.5 57.8 544-558 bp 1.0 78.6