Hotspot

atpH-atpI

LSC · 14676-15828 · 1153 bp

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Pi 0.00185
MarkerSeek score 53.50
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1153
variable_sites
7
pi
0.00185
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 ATAACGGAAGCGGCAGAAAT TTTTGCAACTTTAGCCGCAG 45.0 45.0 58.0 57.9 1230-1240 bp 1.0 79.8
atpH-atpI_p2 TACCTTGACCAACTCCAGGT TTTTGCAACTTTAGCCGCAG 50.0 45.0 57.9 57.9 1286-1296 bp 1.0 79.2
atpH-atpI_p3 AATAACGGAAGCGGCAGAAA TTTTGCAACTTTAGCCGCAG 45.0 45.0 58.2 57.9 1231-1241 bp 1.0 78.6
atpH-atpI_p4 GCAGTACCTTGACCAACTCC TTTTGCAACTTTAGCCGCAG 55.0 45.0 58.2 57.9 1290-1300 bp 1.0 78.6
atpH-atpI_p5 GCCAATCCAGCAGCAATAAC TTTTGCAACTTTAGCCGCAG 50.0 45.0 57.8 57.9 1245-1255 bp 1.0 78.4