Hotspot

rps16-trnQ-UUG

LSC · 6268-7961 · 1694 bp

Back to Schima

Pi 0.00315
MarkerSeek score 55.10
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1694
variable_sites
20
pi
0.00315
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1762-1774 bp 1.0 79.7
rps16-trnQ-UUG_p2 ATCCACAACGAATCGTGTCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.3 58.1 1785-1797 bp 1.0 78.6
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA AAATTAAAATGGGGCGTGGC 45.0 45.0 58.1 57.6 1819-1831 bp 1.0 77.1
rps16-trnQ-UUG_p4 CCACAACGAATCGTGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.5 58.1 1783-1795 bp 1.0 76.9
rps16-trnQ-UUG_p5 CCAAGAGCACCTTCATTCCT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.5 58.1 1827-1839 bp 1.0 76.8