Hotspot

psaC-ndhE

Genome · 96791-96945 · 155 bp

Back to Scapania

Pi 0.114

MarkerSeek score 79.80

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: Genome
length_bp: 155
variable_sites: 16
pi: 0.114
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	AATTTTGACTGAATGTGCCA	AGTCAGCTCGTATTGACCAA	35.0	45.0	54.0	56.6	204-217 bp	1.0	50.1
psaC-ndhE_p2	AATTTTGACTGAATGTGCCA	CGCAATAGAAAGTCAGCTCG	35.0	50.0	54.0	57.0	214-227 bp	1.0	49.8
psaC-ndhE_p3	AAATTTTGACTGAATGTGCCA	CGCAATAGAAAGTCAGCTCG	33.3	50.0	54.8	57.0	215-228 bp	1.0	49.8
psaC-ndhE_p4	ATTTTGACTGAATGTGCCAT	CGCAATAGAAAGTCAGCTCG	35.0	50.0	53.7	57.0	213-226 bp	1.0	49.8
psaC-ndhE_p5	AATTTTGACTGAATGTGCCAT	CGCAATAGAAAGTCAGCTCG	33.3	50.0	54.6	57.0	214-227 bp	1.0	49.8