Hotspot

rpl32-trnL-UAG

Genome · 92728-93563 · 836 bp

Back to Scapania

Pi 0.109
MarkerSeek score 83.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
Genome
length_bp
836
variable_sites
87
pi
0.109
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GAACGGGGAGGAGATGTACT GTAAAGCCGGAACCAAAGGA 55.0 50.0 58.2 58.1 1830 bp 0.5 57.4
rpl32-trnL-UAG_p2 GAACGGGGAGGAGATGTACT GACTGTAAAGCCGGAACCAA 55.0 50.0 58.2 58.1 1834 bp 0.5 57.3
rpl32-trnL-UAG_p3 TTGAACGGGGAGGAGATGTA GTAAAGCCGGAACCAAAGGA 50.0 50.0 57.8 58.1 1832 bp 0.5 57.3
rpl32-trnL-UAG_p4 TTGAACGGGGAGGAGATGTA GACTGTAAAGCCGGAACCAA 50.0 50.0 57.8 58.1 1836 bp 0.5 57.2
rpl32-trnL-UAG_p5 GAACGGGGAGGAGATGTACT GCTTCCTAAGAGCAACGTGT 55.0 50.0 58.2 58.2 1232 bp 0.5 56.6