Hotspot

trnT-UGU-trnL-UAA

Genome · 50247-50512 · 266 bp

Back to Scapania

Pi 0.119

MarkerSeek score 79.00

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: Genome
length_bp: 266
variable_sites: 31
pi: 0.119
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GACCTCTGAGCTAAACAGGC	TTTAAGTCCGCAGCGTCTAC	55.0	50.0	58.0	58.0	324-325 bp	1.0	88.6
trnT-UGU-trnL-UAA_p2	TACAAGTGCGATGCTCTGAC	TTTAAGTCCGCAGCGTCTAC	50.0	50.0	58.0	58.0	341-342 bp	1.0	88.3
trnT-UGU-trnL-UAA_p3	GACCTCTGAGCTAAACAGGC	AATTTAAGTCCGCAGCGTCT	55.0	45.0	58.0	57.9	326-327 bp	1.0	87.9
trnT-UGU-trnL-UAA_p4	TACAAGTGCGATGCTCTGAC	AATTTAAGTCCGCAGCGTCT	50.0	45.0	58.0	57.9	343-344 bp	1.0	87.6
trnT-UGU-trnL-UAA_p5	GCATTACAAGTGCGATGCTC	TTTAAGTCCGCAGCGTCTAC	50.0	50.0	57.9	58.0	345-346 bp	1.0	87.5