Hotspot

trnT-GGU-psbD

Genome · 38050-38658 · 609 bp

Back to Scapania

Pi 0.0643

MarkerSeek score 78.00

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: Genome
length_bp: 609
variable_sites: 38
pi: 0.0643
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	CGGTTCGAATCTGGTAAGGG	TTGCAGCAACACCAAAACTG	55.0	45.0	58.1	58.0	1149-1167 bp	1.0	84.2
trnT-GGU-psbD_p2	GTGGTAGAGTAACGCCATGG	TTGCAGCAACACCAAAACTG	55.0	45.0	58.1	58.0	1184-1202 bp	1.0	84.1
trnT-GGU-psbD_p3	TCAGTGGTAGAGTAACGCCA	TTGCAGCAACACCAAAACTG	50.0	45.0	58.1	58.0	1187-1205 bp	1.0	83.9
trnT-GGU-psbD_p4	CGGTTCGAATCTGGTAAGGG	TGGACCAGAGAATGCGATTG	55.0	50.0	58.1	58.0	1082 bp	0.5	59.3
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	TGGACCAGAGAATGCGATTG	55.0	50.0	58.1	58.0	1117 bp	0.5	59.3