Hotspot

trnE-UUC-trnT-GGU

Genome · 37142-37977 · 836 bp

Back to Scapania

Pi 0.113
MarkerSeek score 80.10
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
Genome
length_bp
836
variable_sites
94
pi
0.113
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 GTCGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.0 58.0 939-946 bp 1.0 88.1
trnE-UUC-trnT-GGU_p2 GTCGCCTCCTTGAAAGAGAG CCCTTACCAGATTCGAACCG 55.0 55.0 58.0 58.1 953-960 bp 1.0 87.6
trnE-UUC-trnT-GGU_p3 GAGGCTTTCATTACCCCCAG GAACCGATGACTTACGCCTT 55.0 50.0 57.9 58.0 975-982 bp 1.0 87.5
trnE-UUC-trnT-GGU_p4 GTCGCCTCCTTGAAAGAGAG GTTGGAAGAGAGGCGTATGG 55.0 55.0 58.0 58.1 1306 bp 0.5 59.5
trnE-UUC-trnT-GGU_p5 GTCGCCTCCTTGAAAGAGAG CCATGGCGTTACTCTACCAC 55.0 55.0 58.0 58.1 918 bp 0.5 59.5