Hotspot

psbM-ycf66

Genome · 4310-4432 · 123 bp

Back to Scapania

Pi 0.102

MarkerSeek score 78.40

Primer available yes

Label psbM-ycf66

Feature Metadata

label_name: psbM-ycf66
feature_type: igs
region: Genome
length_bp: 123
variable_sites: 12
pi: 0.102
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-ycf66_p1	TGTACGAACAGCTGGTCAAA	AGTACTGGGGCCTAATTCCA	45.0	50.0	57.4	57.7	176-181 bp	1.0	80.5
psbM-ycf66_p2	ATGTACGAACAGCTGGTCAA	AGTACTGGGGCCTAATTCCA	45.0	50.0	57.2	57.7	177-182 bp	1.0	78.7
psbM-ycf66_p3	TGTACGAACAGCTGGTCAAAA	AGTACTGGGGCCTAATTCCA	42.9	50.0	58.1	57.7	176-181 bp	1.0	76.6
psbM-ycf66_p4	ATGTACGAACAGCTGGTCAAA	AGTACTGGGGCCTAATTCCA	42.9	50.0	57.9	57.7	177-182 bp	1.0	76.4
psbM-ycf66_p5	TGTACGAACAGCTGGTCAAA	AGTACTGGGGCCTAATTCCAT	45.0	47.6	57.4	58.2	176-181 bp	1.0	73.1