primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps12-rps7_p1	rps12-rps7	1	AATCGGATGTACGGTTTGGG	ATTGGATCAGGCTTCGCAAT	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.095651	997	1046	167-171	1.000000	85.4
rps12-rps7_p2	rps12-rps7	2	AATCGGATGTACGGTTTGGG	CGTGACGTTCGGAGTTACTT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.074784	997	1046	339-343	1.000000	83.6
rps12-rps7_p3	rps12-rps7	3	AATCGGATGTACGGTTTGGG	GATCCACCTATACGACGTGC	20	20	50.0	55.0	58.0	58.0	0.0	11.6	0.080359	997	1046	365-369	1.000000	83.3
rps12-rps7_p4	rps12-rps7	4	AATCGGATGTACGGTTTGGG	GCAGCCATATTTCCACCTGA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.089818	997	1046	470-474	1.000000	83.0
rps12-rps7_p5	rps12-rps7	5	AATCGGATGTACGGTTTGGG	TTACTGATGGCTTGACGTGG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.125987	997	1046	324-324	0.500000	59.0
psbM-ycf66_p1	psbM-ycf66	1	TGTACGAACAGCTGGTCAAA	AGTACTGGGGCCTAATTCCA	20	20	45.0	50.0	57.4	57.7	30.3	0.8	0.886145	4310	4432	176-181	1.000000	80.5
psbM-ycf66_p2	psbM-ycf66	2	ATGTACGAACAGCTGGTCAA	AGTACTGGGGCCTAATTCCA	20	20	45.0	50.0	57.2	57.7	30.3	0.8	1.111993	4310	4432	177-182	1.000000	78.7
psbM-ycf66_p3	psbM-ycf66	3	TGTACGAACAGCTGGTCAAAA	AGTACTGGGGCCTAATTCCA	21	20	42.9	50.0	58.1	57.7	30.3	0.8	1.372674	4310	4432	176-181	1.000000	76.6
psbM-ycf66_p4	psbM-ycf66	4	ATGTACGAACAGCTGGTCAAA	AGTACTGGGGCCTAATTCCA	21	20	42.9	50.0	57.9	57.7	30.3	0.8	1.400629	4310	4432	177-182	1.000000	76.4
psbM-ycf66_p5	psbM-ycf66	5	TGTACGAACAGCTGGTCAAA	AGTACTGGGGCCTAATTCCAT	20	21	45.0	47.6	57.4	58.2	30.3	0.8	1.816618	4310	4432	176-181	1.000000	73.1
trnR-UCU-trnG-UCC_p1	trnR-UCU-trnG-UCC	1	ATCCTTTAGACGATGGACGC	ACCCTTAGCCTTCCAAGCTA	20	20	50.0	50.0	57.5	58.0	0.0	0.0	0.543922	21524	21600	145-147	1.000000	81.1
trnR-UCU-trnG-UCC_p2	trnR-UCU-trnG-UCC	2	ATCCTTTAGACGATGGACGC	TTTGCATGTTCGGTTCTGGA	20	20	50.0	45.0	57.5	58.0	0.0	0.0	0.536503	21524	21600	203-205	1.000000	79.6
trnR-UCU-trnG-UCC_p3	trnR-UCU-trnG-UCC	3	ATCCTTTAGACGATGGACGC	TTCCAAGCTAATGATGCGGG	20	20	50.0	50.0	57.5	58.3	0.0	0.0	0.831629	21524	21600	135-137	1.000000	79.2
trnR-UCU-trnG-UCC_p4	trnR-UCU-trnG-UCC	4	ATCCTTTAGACGATGGACGC	TCGGTTCTGGAGTAGAGACG	20	20	50.0	55.0	57.5	58.3	0.0	0.0	0.775381	21524	21600	194-196	1.000000	77.9
trnR-UCU-trnG-UCC_p5	trnR-UCU-trnG-UCC	5	ATCCTTTAGACGATGGACGC	GCATGTTCGGTTCTGGAGTA	20	20	50.0	50.0	57.5	57.6	0.0	0.0	0.890851	21524	21600	200-202	1.000000	76.9
chlB-trnK-UUU_p1	chlB-trnK-UUU	1	GACAAAACGGTGTTGCCAAA	CAAGTCGCACTTAAAAGCCG	20	20	45.0	50.0	58.0	58.0	10.9	1.9	0.051859	25710	26216	587-622	1.000000	87.7
chlB-trnK-UUU_p2	chlB-trnK-UUU	2	GACAAAACGGTGTTGCCAAA	AAGTCGCACTTAAAAGCCGA	20	20	45.0	45.0	58.0	58.1	10.9	0.4	0.128633	25710	26216	586-621	1.000000	87.1
chlB-trnK-UUU_p3	chlB-trnK-UUU	3	CGGAAAAATTTGCCCGACAA	CAAGTCGCACTTAAAAGCCG	20	20	45.0	50.0	57.9	58.0	20.5	1.9	0.191064	25710	26216	602-637	1.000000	86.5
chlB-trnK-UUU_p4	chlB-trnK-UUU	4	CGGAAAAATTTGCCCGACAA	AAGTCGCACTTAAAAGCCGA	20	20	45.0	45.0	57.9	58.1	20.5	0.4	0.267837	25710	26216	601-636	1.000000	85.9
chlB-trnK-UUU_p5	chlB-trnK-UUU	5	GACAAAACGGTGTTGCCAAA	CCAAGTCGCACTTAAAAGCC	20	20	45.0	50.0	58.0	57.7	10.9	1.9	0.334140	25710	26216	588-623	1.000000	85.4
trnH-GUG-ycf2_p1	trnH-GUG-ycf2	1	GCGCTAACCTTGGTATGGAA	AACCCGAGTAAACTGCGTTT	20	20	50.0	45.0	58.0	58.0	0.0	10.6	0.075012	29771	30569	1202-1207	1.000000	85.9
trnH-GUG-ycf2_p2	trnH-GUG-ycf2	2	GCGCTAACCTTGGTATGGAA	ACCCGAGTAAACTGCGTTTT	20	20	50.0	45.0	58.0	58.0	0.0	11.0	0.075012	29771	30569	1201-1206	1.000000	85.9
trnH-GUG-ycf2_p3	trnH-GUG-ycf2	3	TGTTGCAGCTCACGGTTATT	ACCCGAGTAAACTGCGTTTT	20	20	45.0	45.0	58.1	58.0	1.2	11.0	0.151456	29771	30569	1425-1430	1.000000	84.4
trnH-GUG-ycf2_p4	trnH-GUG-ycf2	4	TGGGTGTTGCTGGTGTATTC	AACCCGAGTAAACTGCGTTT	20	20	50.0	45.0	58.1	58.0	0.0	10.6	0.138045	29771	30569	1571-1576	1.000000	84.0
trnH-GUG-ycf2_p5	trnH-GUG-ycf2	5	TGGGTGTTGCTGGTGTATTC	ACCCGAGTAAACTGCGTTTT	20	20	50.0	45.0	58.1	58.0	0.0	11.0	0.138045	29771	30569	1570-1575	1.000000	84.0
ycf2_p1	ycf2	1	TTTCCCTTTAACCGCGCATA	CCCACCATTCCCATGTAAAGA	20	21	45.0	47.6	57.9	57.6	8.7	0.0	1.491372	32744	32863	1519-1519	0.500000	48.1
ycf2_p2	ycf2	2	TTTCCCTTTAACCGCGCATA	TCCCACCATTCCCATGTAAAG	20	21	45.0	47.6	57.9	57.6	8.7	0.0	1.491372	32744	32863	1520-1520	0.500000	48.1
ycf2_p3	ycf2	3	TTCCCTTTAACCGCGCATAT	CCCACCATTCCCATGTAAAGA	20	21	45.0	47.6	57.7	57.6	8.7	0.0	1.715787	32744	32863	1518-1518	0.500000	46.3
ycf2_p4	ycf2	4	TTCCCTTTAACCGCGCATAT	TCCCACCATTCCCATGTAAAG	20	21	45.0	47.6	57.7	57.6	8.7	0.0	1.715787	32744	32863	1519-1519	0.500000	46.3
ycf2_p5	ycf2	5	TTTCCCTTTAACCGCGCATA	TCCCACCATTCCCATGTAAA	20	20	45.0	45.0	57.9	56.4	8.7	0.0	1.719856	32744	32863	1520-1520	0.500000	46.2
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	GTCGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.009167	37142	37977	939-946	1.000000	88.1
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	GTCGCCTCCTTGAAAGAGAG	CCCTTACCAGATTCGAACCG	20	20	55.0	55.0	58.0	58.1	0.0	0.0	0.067264	37142	37977	953-960	1.000000	87.6
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	GAGGCTTTCATTACCCCCAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.064563	37142	37977	975-982	1.000000	87.5
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	GTCGCCTCCTTGAAAGAGAG	GTTGGAAGAGAGGCGTATGG	20	20	55.0	55.0	58.0	58.1	0.0	0.0	0.061871	37142	37977	1306-1306	0.500000	59.5
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	GTCGCCTCCTTGAAAGAGAG	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	58.0	58.1	0.0	0.0	0.067264	37142	37977	918-918	0.500000	59.5
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	CGGTTCGAATCTGGTAAGGG	TTGCAGCAACACCAAAACTG	20	20	55.0	45.0	58.1	58.0	6.9	2.8	0.071428	38050	38658	1149-1167	1.000000	84.2
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	GTGGTAGAGTAACGCCATGG	TTGCAGCAACACCAAAACTG	20	20	55.0	45.0	58.1	58.0	7.6	2.8	0.071428	38050	38658	1184-1202	1.000000	84.1
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	TCAGTGGTAGAGTAACGCCA	TTGCAGCAACACCAAAACTG	20	20	50.0	45.0	58.1	58.0	0.0	2.8	0.093582	38050	38658	1187-1205	1.000000	83.9
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	CGGTTCGAATCTGGTAAGGG	TGGACCAGAGAATGCGATTG	20	20	55.0	50.0	58.1	58.0	6.9	0.0	0.092487	38050	38658	1082-1082	0.500000	59.3
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GTGGTAGAGTAACGCCATGG	TGGACCAGAGAATGCGATTG	20	20	55.0	50.0	58.1	58.0	7.6	0.0	0.092487	38050	38658	1117-1117	0.500000	59.3
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GACCTCTGAGCTAAACAGGC	TTTAAGTCCGCAGCGTCTAC	20	20	55.0	50.0	58.0	58.0	0.0	15.0	0.030440	50247	50512	324-325	1.000000	88.6
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	TACAAGTGCGATGCTCTGAC	TTTAAGTCCGCAGCGTCTAC	20	20	50.0	50.0	58.0	58.0	0.0	15.0	0.017010	50247	50512	341-342	1.000000	88.3
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GACCTCTGAGCTAAACAGGC	AATTTAAGTCCGCAGCGTCT	20	20	55.0	45.0	58.0	57.9	0.0	15.0	0.111900	50247	50512	326-327	1.000000	87.9
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	TACAAGTGCGATGCTCTGAC	AATTTAAGTCCGCAGCGTCT	20	20	50.0	45.0	58.0	57.9	0.0	15.0	0.098471	50247	50512	343-344	1.000000	87.6
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCATTACAAGTGCGATGCTC	TTTAAGTCCGCAGCGTCTAC	20	20	50.0	50.0	57.9	58.0	10.5	15.0	0.110101	50247	50512	345-346	1.000000	87.5
trnL-UAA-trnF-GAA_p1	trnL-UAA-trnF-GAA	1	CGTTGGCTTTTTAGACCGTG	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	57.7	58.1	0.0	20.1	0.361793	50897	50965	172-172	1.000000	80.4
trnL-UAA-trnF-GAA_p2	trnL-UAA-trnF-GAA	2	TCCGTTGGCTTTTTAGACCG	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	58.5	58.1	0.0	20.1	0.536814	50897	50965	174-174	1.000000	78.9
trnL-UAA-trnF-GAA_p3	trnL-UAA-trnF-GAA	3	CGTTGGCTTTTTAGACCGTG	GAACTGGTGACACGAGGATT	20	20	50.0	50.0	57.7	57.5	0.0	20.1	0.767264	50897	50965	169-169	1.000000	77.2
trnL-UAA-trnF-GAA_p4	trnL-UAA-trnF-GAA	4	CGTTGGCTTTTTAGACCGTG	TACCAACTGAGCTATCCCGG	20	20	50.0	55.0	57.7	58.6	0.0	0.0	0.899893	50897	50965	134-134	1.000000	77.0
trnL-UAA-trnF-GAA_p5	trnL-UAA-trnF-GAA	5	CCGTTGGCTTTTTAGACCGT	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	58.8	58.1	0.0	20.1	0.819776	50897	50965	173-173	1.000000	76.7
atpB-rbcL_p1	atpB-rbcL	1	TCTTGCCAGGTGAAAAAGCA	GCTTTGAATCCAACACCTGC	20	20	45.0	50.0	58.2	57.9	0.0	0.0	0.296152	55829	56390	702-718	1.000000	83.3
atpB-rbcL_p2	atpB-rbcL	2	TCTTGCCAGGTGAAAAAGCA	GTCTCCGTTTGTGGTGACAT	20	20	45.0	50.0	58.2	58.1	0.0	9.4	0.346937	55829	56390	678-694	1.000000	83.1
atpB-rbcL_p3	atpB-rbcL	3	TTGCCAGGTGAAAAAGCAAC	GCTTTGAATCCAACACCTGC	20	20	45.0	50.0	57.7	57.9	0.0	0.0	0.378195	55829	56390	700-716	1.000000	82.6
atpB-rbcL_p4	atpB-rbcL	4	TTGCCAGGTGAAAAAGCAAC	GTCTCCGTTTGTGGTGACAT	20	20	45.0	50.0	57.7	58.1	0.0	9.4	0.428981	55829	56390	676-692	1.000000	82.4
atpB-rbcL_p5	atpB-rbcL	5	GCCAGGTGAAAAAGCAACAT	GCTTTGAATCCAACACCTGC	20	20	45.0	50.0	57.5	57.9	0.0	0.0	0.601784	55829	56390	698-714	1.000000	80.9
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GAACGGGGAGGAGATGTACT	GTAAAGCCGGAACCAAAGGA	20	20	55.0	50.0	58.2	58.1	0.0	5.0	0.321814	92728	93563	1830-1830	0.500000	57.4
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GAACGGGGAGGAGATGTACT	GACTGTAAAGCCGGAACCAA	20	20	55.0	50.0	58.2	58.1	0.0	5.0	0.335173	92728	93563	1834-1834	0.500000	57.3
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	TTGAACGGGGAGGAGATGTA	GTAAAGCCGGAACCAAAGGA	20	20	50.0	50.0	57.8	58.1	0.0	5.0	0.341035	92728	93563	1832-1832	0.500000	57.3
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TTGAACGGGGAGGAGATGTA	GACTGTAAAGCCGGAACCAA	20	20	50.0	50.0	57.8	58.1	0.0	5.0	0.354394	92728	93563	1836-1836	0.500000	57.2
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GAACGGGGAGGAGATGTACT	GCTTCCTAAGAGCAACGTGT	20	20	55.0	50.0	58.2	58.2	0.0	0.0	0.425035	92728	93563	1232-1232	0.500000	56.6
psaC-ndhE_p1	psaC-ndhE	1	AATTTTGACTGAATGTGCCA	AGTCAGCTCGTATTGACCAA	20	20	35.0	45.0	54.0	56.6	0.0	10.7	5.467280	96791	96945	204-217	1.000000	50.1
psaC-ndhE_p2	psaC-ndhE	2	AATTTTGACTGAATGTGCCA	CGCAATAGAAAGTCAGCTCG	20	20	35.0	50.0	54.0	57.0	0.0	0.0	5.034245	96791	96945	214-227	1.000000	49.8
psaC-ndhE_p3	psaC-ndhE	3	AAATTTTGACTGAATGTGCCA	CGCAATAGAAAGTCAGCTCG	21	20	33.3	50.0	54.8	57.0	0.0	0.0	5.163960	96791	96945	215-228	1.000000	49.8
psaC-ndhE_p4	psaC-ndhE	4	ATTTTGACTGAATGTGCCAT	CGCAATAGAAAGTCAGCTCG	20	20	35.0	50.0	53.7	57.0	0.0	0.0	5.276752	96791	96945	213-226	1.000000	49.8
psaC-ndhE_p5	psaC-ndhE	5	AATTTTGACTGAATGTGCCAT	CGCAATAGAAAGTCAGCTCG	21	20	33.3	50.0	54.6	57.0	0.0	0.0	5.391697	96791	96945	214-227	1.000000	49.8