Hotspot

ccsA

SSC · 115345-116316 · 972 bp

Back to Scabiosa

Pi 0.478

MarkerSeek score 80.50

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 389
pi: 0.478
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	AGGTTCTCGCCAATTCGTAC	GTGAAATCGGTAGACACGCT	50.0	50.0	58.0	58.0	1231 bp	0.5	59.9
ccsA_p2	AGGTTCTCGCCAATTCGTAC	TGAAATCGGTAGACACGCTG	50.0	50.0	58.0	58.0	1230 bp	0.5	59.9
ccsA_p3	AGGTCGAACCAAACCAAGAC	GTGAAATCGGTAGACACGCT	50.0	50.0	58.0	58.0	1597 bp	0.5	59.6
ccsA_p4	AGGTCGAACCAAACCAAGAC	TGAAATCGGTAGACACGCTG	50.0	50.0	58.0	58.0	1596 bp	0.5	59.6
ccsA_p5	TCCCCCTTGGAGGAAGAAAT	GTGAAATCGGTAGACACGCT	50.0	50.0	57.9	58.0	1631 bp	0.5	59.3