Hotspot

ycf1-trnN-GUU

IRa · 133973-134443 · 471 bp

Back to Scabiosa

Pi 0.432

MarkerSeek score 77.20

Primer available yes

Label ycf1-trnN-GUU

Feature Metadata

label_name: ycf1-trnN-GUU
feature_type: igs
region: IRa
length_bp: 471
variable_sites: 160
pi: 0.432
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnN-GUU_p1	GCTGAAACTGCGCATTGAAT	AACCTACACCGAGGGATTGA	45.0	50.0	58.0	58.1	1034-1687 bp	1.0	91.5
ycf1-trnN-GUU_p2	GCTGAAACTGCGCATTGAAT	GCCTGTAGCTCAGAGGAGTA	45.0	55.0	58.0	57.9	1134-1787 bp	1.0	91.4
ycf1-trnN-GUU_p3	GAGTGCGGTCAGAAATCCAT	CCTCGCAATCACTAGCCAAT	50.0	50.0	58.0	58.0	1284-2290 bp	1.0	90.8
ycf1-trnN-GUU_p4	GCTGAAACTGCGCATTGAAT	GGTGACGATACTGTAGGGGA	45.0	55.0	58.0	57.9	1448-2454 bp	1.0	90.2
ycf1-trnN-GUU_p5	GCTGAAACTGCGCATTGAAT	CCTCGCAATCACTAGCCAAT	45.0	50.0	58.0	58.0	1575-2581 bp	1.0	90.0