Hotspot

trnL-UAG-rpl32

SSC · 116507-117310 · 804 bp

Back to Scabiosa

Pi 0.463

MarkerSeek score 81.00

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: SSC
length_bp: 804
variable_sites: 319
pi: 0.463
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	TATCACGCCCTTTTCTGACG	AGAGGATTGGCGGAATTGAC	50.0	50.0	58.0	58.0	1663 bp	0.5	59.6
trnL-UAG-rpl32_p2	TATCACGCCCTTTTCTGACG	AGGATTGGCGGAATTGACTC	50.0	50.0	58.0	58.0	1661 bp	0.5	59.6
trnL-UAG-rpl32_p3	TATCACGCCCTTTTCTGACG	GAGGATTGGCGGAATTGACT	50.0	50.0	58.0	58.0	1662 bp	0.5	59.6
trnL-UAG-rpl32_p4	AGCGTGTCTACCGATTTCAC	AGGATTGGCGGAATTGACTC	50.0	50.0	58.0	58.0	1362 bp	0.5	59.6
trnL-UAG-rpl32_p5	AGCGTGTCTACCGATTTCAC	GAGGATTGGCGGAATTGACT	50.0	50.0	58.0	58.0	1363 bp	0.5	59.6