Hotspot

ycf1-ccsA

SSC · 114207-115344 · 1138 bp

Back to Scabiosa

Pi 0.454

MarkerSeek score 74.00

Primer available yes

Label ycf1-ccsA

Feature Metadata

label_name: ycf1-ccsA
feature_type: igs
region: SSC
length_bp: 1138
variable_sites: 295
pi: 0.454
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.57
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ccsA_p1	GAGCTTACGAACTGAAGGGG	ATAACTGCGTCAAGTGCGAT	55.0	45.0	58.0	58.0	1920 bp	0.5	59.8
ycf1-ccsA_p2	GGAGCTTACGAACTGAAGGG	ATAACTGCGTCAAGTGCGAT	55.0	45.0	58.0	58.0	1921 bp	0.5	59.8
ycf1-ccsA_p3	ATTCAATGCGCAGTTTCAGC	ATAACTGCGTCAAGTGCGAT	45.0	45.0	58.0	58.0	2466 bp	0.5	59.8
ycf1-ccsA_p4	TCCCCCGAAGAAGATCCTTT	ATAACTGCGTCAAGTGCGAT	50.0	45.0	58.0	58.0	1869 bp	0.5	59.7
ycf1-ccsA_p5	TTCCCCCGAAGAAGATCCTT	ATAACTGCGTCAAGTGCGAT	50.0	45.0	58.0	58.0	1870 bp	0.5	59.7