Hotspot

trnN-GUU-ycf1

IRb · 112686-113156 · 471 bp

Back to Scabiosa

Pi 0.204
MarkerSeek score 86.90
Primer available yes
Label trnN-GUU-ycf1

Feature Metadata

label_name
trnN-GUU-ycf1
feature_type
igs
region
IRb
length_bp
471
variable_sites
69
pi
0.204
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.72
markerseek_score
86.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-ycf1_p1 AGGATTCGAACCTACGACCA TCCATAATAGAGCCCGACCA 50.0 50.0 58.2 57.6 641 bp 0.5 55.6
trnN-GUU-ycf1_p2 GGATTCGAACCTACGACCAG TCCATAATAGAGCCCGACCA 55.0 50.0 57.8 57.6 640 bp 0.5 55.2
trnN-GUU-ycf1_p3 AGGATTCGAACCTACGACCA CCATAATAGAGCCCGACCAC 50.0 55.0 58.2 57.5 640 bp 0.5 55.1
trnN-GUU-ycf1_p4 GGATTCGAACCTACGACCAG CCATAATAGAGCCCGACCAC 55.0 55.0 57.8 57.5 639 bp 0.5 54.7
trnN-GUU-ycf1_p5 CCCCAAGTAGGATTCGAACC TCCATAATAGAGCCCGACCA 55.0 50.0 57.7 57.6 649 bp 0.5 54.2