Hotspot

rbcL-accD

LSC · 58966-59802 · 837 bp

Back to Scabiosa

Pi 0.133

MarkerSeek score 81.40

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 837
variable_sites: 97
pi: 0.133
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	AATGAGGGACGTGATCTTGC	AACCCGTCCTCAGTAGTCAA	50.0	50.0	58.0	58.0	1469 bp	0.5	59.7
rbcL-accD_p2	AATGAGGGACGTGATCTTGC	ACCCGTCCTCAGTAGTCAAA	50.0	50.0	58.0	58.0	1468 bp	0.5	59.7
rbcL-accD_p3	AATGAGGGACGTGATCTTGC	CATCGAAGGCAGTGGAATCA	50.0	50.0	58.0	58.0	1069 bp	0.5	59.5
rbcL-accD_p4	AGGGCTGTCTTTGCTAGAGA	AACCCGTCCTCAGTAGTCAA	50.0	50.0	58.1	58.0	2006 bp	0.5	59.5
rbcL-accD_p5	AGGGCTGTCTTTGCTAGAGA	ACCCGTCCTCAGTAGTCAAA	50.0	50.0	58.1	58.0	2005 bp	0.5	59.5