primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	atpA-atpF	1	CCTACTTGAAGTACGGTGCC	TTGGGAGCGATGAACGAAAT	20	20	55.0	45.0	58.0	57.9	0.0	0.0	0.113921	12817	12893	214-219	1.000000	80.2
atpA-atpF_p2	atpA-atpF	2	CCTACTTGAAGTACGGTGCC	GGCGCTCTAGGAACTTTGAA	20	20	55.0	50.0	58.0	57.9	0.0	7.6	0.100733	12817	12893	289-294	1.000000	80.0
atpA-atpF_p3	atpA-atpF	3	CCTACTTGAAGTACGGTGCC	AAGGCGCTCTAGGAACTTTG	20	20	55.0	50.0	58.0	57.9	0.0	7.6	0.106575	12817	12893	291-296	1.000000	80.0
atpA-atpF_p4	atpA-atpF	4	CCTACTTGAAGTACGGTGCC	CAAGGCGCTCTAGGAACTTT	20	20	55.0	50.0	58.0	57.9	0.0	7.6	0.106575	12817	12893	292-297	1.000000	80.0
atpA-atpF_p5	atpA-atpF	5	GAAGTACGGTGCCGGTATTT	GGCGCTCTAGGAACTTTGAA	20	20	50.0	50.0	58.0	57.9	15.4	7.6	0.109098	12817	12893	282-287	1.000000	80.0
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.9	57.8	5.2	0.0	0.336606	52602	53606	1136-1204	1.000000	84.8
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	CCTTTGCTAATCGGGGCTAA	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.7	57.8	0.0	0.0	0.539129	52602	53606	1148-1216	1.000000	83.1
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	GGGGCTAAAACTCCGGAAAT	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	57.9	57.5	5.2	0.0	0.680379	52602	53606	1135-1203	1.000000	82.0
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TAATCGGGGCTAAAACTCCG	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.4	57.8	0.0	0.0	0.804914	52602	53606	1141-1209	1.000000	81.0
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	GGGGCTAAAACTCCGGAAAT	GTTCGAGTCCGTATAGCCCT	20	20	50.0	55.0	57.9	58.7	5.2	0.0	0.822672	52602	53606	1124-1192	1.000000	80.9
rbcL-accD_p1	rbcL-accD	1	AATGAGGGACGTGATCTTGC	AACCCGTCCTCAGTAGTCAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.035208	58966	59802	1469-1469	0.500000	59.7
rbcL-accD_p2	rbcL-accD	2	AATGAGGGACGTGATCTTGC	ACCCGTCCTCAGTAGTCAAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.035208	58966	59802	1468-1468	0.500000	59.7
rbcL-accD_p3	rbcL-accD	3	AATGAGGGACGTGATCTTGC	CATCGAAGGCAGTGGAATCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.058258	58966	59802	1069-1069	0.500000	59.5
rbcL-accD_p4	rbcL-accD	4	AGGGCTGTCTTTGCTAGAGA	AACCCGTCCTCAGTAGTCAA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.061193	58966	59802	2006-2006	0.500000	59.5
rbcL-accD_p5	rbcL-accD	5	AGGGCTGTCTTTGCTAGAGA	ACCCGTCCTCAGTAGTCAAA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.061193	58966	59802	2005-2005	0.500000	59.5
accD_p1	accD	1	TTTCTACCCGCGCTTTCTAC	TATACGTAAGGCGGGAGGAG	20	20	50.0	55.0	58.0	58.1	5.6	0.0	0.098838	59803	61644	2656-2656	0.500000	59.2
accD_p2	accD	2	TTTCTACCCGCGCTTTCTAC	CGGGAGGAGGAAATTCGTTT	20	20	50.0	50.0	58.0	57.9	5.6	0.0	0.109854	59803	61644	2645-2645	0.500000	59.1
accD_p3	accD	3	TCTACCCGCGCTTTCTACTA	TATACGTAAGGCGGGAGGAG	20	20	50.0	55.0	58.0	58.1	5.6	0.0	0.141095	59803	61644	2654-2654	0.500000	58.9
accD_p4	accD	4	TCTACCCGCGCTTTCTACTA	CGGGAGGAGGAAATTCGTTT	20	20	50.0	50.0	58.0	57.9	5.6	0.0	0.152111	59803	61644	2643-2643	0.500000	58.8
accD_p5	accD	5	AGTTTCCCTGGATCGAGACA	TATACGTAAGGCGGGAGGAG	20	20	50.0	55.0	58.1	58.1	6.3	0.0	0.159825	59803	61644	2694-2694	0.500000	58.7
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.9	57.1	6.4	0.0	0.992787	68048	69356	1453-1501	1.000000	84.1
psbE-petL_p2	psbE-petL	2	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.8	57.1	24.9	0.0	1.097796	68048	69356	1376-1424	1.000000	83.2
psbE-petL_p3	psbE-petL	3	ACGAATAACCAACCCGCAAT	GTCGTATCTTGCTCAGACCA	20	20	45.0	50.0	58.2	57.1	0.0	0.0	1.112474	68048	69356	1462-1510	1.000000	83.1
psbE-petL_p4	psbE-petL	4	TGCTGACGAATAACCAACCC	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	58.2	57.1	0.0	0.0	1.125378	68048	69356	1467-1515	1.000000	83.0
psbE-petL_p5	psbE-petL	5	CGAATAACCAACCCGCAATG	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.8	57.1	0.0	0.0	1.128804	68048	69356	1461-1509	1.000000	83.0
infA-rps8_p1	infA-rps8	1	ACTCGGAACATACCATTGGG	CGGGAAGCTCGACTAGAAAG	20	20	50.0	55.0	57.3	57.8	7.8	0.0	0.906391	84177	84291	237-237	1.000000	74.4
infA-rps8_p2	infA-rps8	2	ACTCGGAACATACCATTGGG	TCGGCGGAGAAATTTTGTGT	20	20	50.0	45.0	57.3	58.4	7.8	7.2	1.110073	84177	84291	215-215	1.000000	73.0
infA-rps8_p3	infA-rps8	3	ACTCGGAACATACCATTGGG	CGGCGGAGAAATTTTGTGTT	20	20	50.0	45.0	57.3	57.6	7.8	0.0	1.125793	84177	84291	214-214	1.000000	72.9
infA-rps8_p4	infA-rps8	4	ACTCGGAACATACCATTGGG	AGAATTTTAGGCGGGATGGG	20	20	50.0	50.0	57.3	57.6	7.8	0.0	1.075068	84177	84291	297-297	1.000000	72.7
infA-rps8_p5	infA-rps8	5	ACTCGGAACATACCATTGGG	CGGGATGGGGATTGTCATTC	20	20	50.0	55.0	57.3	58.4	7.8	0.0	1.100888	84177	84291	286-286	1.000000	72.6
trnN-GUU-ycf1_p1	trnN-GUU-ycf1	1	AGGATTCGAACCTACGACCA	TCCATAATAGAGCCCGACCA	20	20	50.0	50.0	58.2	57.6	15.0	0.0	0.548558	112686	113156	641-641	0.500000	55.6
trnN-GUU-ycf1_p2	trnN-GUU-ycf1	2	GGATTCGAACCTACGACCAG	TCCATAATAGAGCCCGACCA	20	20	55.0	50.0	57.8	57.6	0.0	0.0	0.597233	112686	113156	640-640	0.500000	55.2
trnN-GUU-ycf1_p3	trnN-GUU-ycf1	3	AGGATTCGAACCTACGACCA	CCATAATAGAGCCCGACCAC	20	20	50.0	55.0	58.2	57.5	15.0	0.0	0.613998	112686	113156	640-640	0.500000	55.1
trnN-GUU-ycf1_p4	trnN-GUU-ycf1	4	GGATTCGAACCTACGACCAG	CCATAATAGAGCCCGACCAC	20	20	55.0	55.0	57.8	57.5	0.0	0.0	0.662673	112686	113156	639-639	0.500000	54.7
trnN-GUU-ycf1_p5	trnN-GUU-ycf1	5	CCCCAAGTAGGATTCGAACC	TCCATAATAGAGCCCGACCA	20	20	55.0	50.0	57.7	57.6	0.0	0.0	0.719126	112686	113156	649-649	0.500000	54.2
ycf1-ccsA_p1	ycf1-ccsA	1	GAGCTTACGAACTGAAGGGG	ATAACTGCGTCAAGTGCGAT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.021685	114207	115344	1920-1920	0.500000	59.8
ycf1-ccsA_p2	ycf1-ccsA	2	GGAGCTTACGAACTGAAGGG	ATAACTGCGTCAAGTGCGAT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.021685	114207	115344	1921-1921	0.500000	59.8
ycf1-ccsA_p3	ycf1-ccsA	3	ATTCAATGCGCAGTTTCAGC	ATAACTGCGTCAAGTGCGAT	20	20	45.0	45.0	58.0	58.0	18.3	0.0	0.023447	114207	115344	2466-2466	0.500000	59.8
ycf1-ccsA_p4	ycf1-ccsA	4	TCCCCCGAAGAAGATCCTTT	ATAACTGCGTCAAGTGCGAT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.043300	114207	115344	1869-1869	0.500000	59.7
ycf1-ccsA_p5	ycf1-ccsA	5	TTCCCCCGAAGAAGATCCTT	ATAACTGCGTCAAGTGCGAT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.043300	114207	115344	1870-1870	0.500000	59.7
ccsA_p1	ccsA	1	AGGTTCTCGCCAATTCGTAC	GTGAAATCGGTAGACACGCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.013801	115345	116316	1231-1231	0.500000	59.9
ccsA_p2	ccsA	2	AGGTTCTCGCCAATTCGTAC	TGAAATCGGTAGACACGCTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.015171	115345	116316	1230-1230	0.500000	59.9
ccsA_p3	ccsA	3	AGGTCGAACCAAACCAAGAC	GTGAAATCGGTAGACACGCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.044658	115345	116316	1597-1597	0.500000	59.6
ccsA_p4	ccsA	4	AGGTCGAACCAAACCAAGAC	TGAAATCGGTAGACACGCTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.046029	115345	116316	1596-1596	0.500000	59.6
ccsA_p5	ccsA	5	TCCCCCTTGGAGGAAGAAAT	GTGAAATCGGTAGACACGCT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.082753	115345	116316	1631-1631	0.500000	59.3
trnL-UAG_p1	trnL-UAG	1	TATCACGCCCTTTTCTGACG	TGAACTCATCGCATCCTGTT	20	20	50.0	45.0	58.0	57.2	0.0	0.0	0.772836	116427	116506	440-440	0.500000	53.8
trnL-UAG_p2	trnL-UAG	2	TATCACGCCCTTTTCTGACG	TTGAACTCATCGCATCCTGT	20	20	50.0	45.0	58.0	57.2	0.0	0.0	0.772836	116427	116506	441-441	0.500000	53.8
trnL-UAG_p3	trnL-UAG	3	GCTATCACGCCCTTTTCTGA	TGAACTCATCGCATCCTGTT	20	20	50.0	45.0	58.0	57.2	0.0	0.0	0.792429	116427	116506	442-442	0.500000	53.7
trnL-UAG_p4	trnL-UAG	4	GCTATCACGCCCTTTTCTGA	TTGAACTCATCGCATCCTGT	20	20	50.0	45.0	58.0	57.2	0.0	0.0	0.792429	116427	116506	443-443	0.500000	53.7
trnL-UAG_p5	trnL-UAG	5	AAAAATCGCACTTGACGCAG	TGAACTCATCGCATCCTGTT	20	20	45.0	45.0	58.0	57.2	0.0	0.0	0.804809	116427	116506	626-626	0.500000	53.6
trnL-UAG-rpl32_p1	trnL-UAG-rpl32	1	TATCACGCCCTTTTCTGACG	AGAGGATTGGCGGAATTGAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.048705	116507	117310	1663-1663	0.500000	59.6
trnL-UAG-rpl32_p2	trnL-UAG-rpl32	2	TATCACGCCCTTTTCTGACG	AGGATTGGCGGAATTGACTC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.048705	116507	117310	1661-1661	0.500000	59.6
trnL-UAG-rpl32_p3	trnL-UAG-rpl32	3	TATCACGCCCTTTTCTGACG	GAGGATTGGCGGAATTGACT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.048705	116507	117310	1662-1662	0.500000	59.6
trnL-UAG-rpl32_p4	trnL-UAG-rpl32	4	AGCGTGTCTACCGATTTCAC	AGGATTGGCGGAATTGACTC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.051953	116507	117310	1362-1362	0.500000	59.6
trnL-UAG-rpl32_p5	trnL-UAG-rpl32	5	AGCGTGTCTACCGATTTCAC	GAGGATTGGCGGAATTGACT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.051953	116507	117310	1363-1363	0.500000	59.6
rpl32_p1	rpl32	1	AACAGGATGCGATGAGTTCA	AGGATTGGCGGAATTGACTC	20	20	45.0	50.0	57.2	58.0	0.0	0.0	0.812376	117311	117481	1241-1241	0.500000	53.5
rpl32_p2	rpl32	2	AACAGGATGCGATGAGTTCA	GAGGATTGGCGGAATTGACT	20	20	45.0	50.0	57.2	58.0	0.0	0.0	0.812376	117311	117481	1242-1242	0.500000	53.5
rpl32_p3	rpl32	3	ACAGGATGCGATGAGTTCAA	AGAGGATTGGCGGAATTGAC	20	20	45.0	50.0	57.2	58.0	0.0	0.0	0.812376	117311	117481	1242-1242	0.500000	53.5
rpl32_p4	rpl32	4	ACAGGATGCGATGAGTTCAA	AGGATTGGCGGAATTGACTC	20	20	45.0	50.0	57.2	58.0	0.0	0.0	0.812376	117311	117481	1240-1240	0.500000	53.5
rpl32_p5	rpl32	5	ACAGGATGCGATGAGTTCAA	GAGGATTGGCGGAATTGACT	20	20	45.0	50.0	57.2	58.0	0.0	0.0	0.812376	117311	117481	1241-1241	0.500000	53.5
rpl32-ndhF_p1	rpl32-ndhF	1	CAAAGCTTTTAACGCGGACC	AGAGGATTGGCGGAATTGAC	20	20	50.0	50.0	58.3	58.0	12.5	0.0	0.362298	117482	117619	456-456	0.500000	57.1
rpl32-ndhF_p2	rpl32-ndhF	2	CAAAGCTTTTAACGCGGACC	AGGATTGGCGGAATTGACTC	20	20	50.0	50.0	58.3	58.0	12.5	0.0	0.362298	117482	117619	454-454	0.500000	57.1
rpl32-ndhF_p3	rpl32-ndhF	3	CAAAGCTTTTAACGCGGACC	GAGGATTGGCGGAATTGACT	20	20	50.0	50.0	58.3	58.0	12.5	0.0	0.362298	117482	117619	455-455	0.500000	57.1
rpl32-ndhF_p4	rpl32-ndhF	4	TAACGCGGACCAATATGCTT	AGGATTGGCGGAATTGACTC	20	20	45.0	50.0	57.7	58.0	12.5	0.0	0.363259	117482	117619	445-445	0.500000	57.1
rpl32-ndhF_p5	rpl32-ndhF	5	TAACGCGGACCAATATGCTT	GAGGATTGGCGGAATTGACT	20	20	45.0	50.0	57.7	58.0	12.5	0.0	0.363259	117482	117619	446-446	0.500000	57.1
ndhF_p1	ndhF	1	CAAAGCTTTTAACGCGGACC	TGGACCGCGAGAGTTATTTG	20	20	50.0	50.0	58.3	58.0	12.5	8.2	0.322758	117620	119887	2755-2755	0.500000	57.4
ndhF_p2	ndhF	2	TAACGCGGACCAATATGCTT	TGGACCGCGAGAGTTATTTG	20	20	45.0	50.0	57.7	58.0	12.5	8.2	0.323719	117620	119887	2746-2746	0.500000	57.4
ndhF_p3	ndhF	3	TTAACGCGGACCAATATGCT	TGGACCGCGAGAGTTATTTG	20	20	45.0	50.0	57.7	58.0	12.5	8.2	0.323719	117620	119887	2747-2747	0.500000	57.4
ndhF_p4	ndhF	4	CAAAGCTTTTAACGCGGACC	GGACCGCGAGAGTTATTTGT	20	20	50.0	50.0	58.3	58.0	12.5	8.2	0.324146	117620	119887	2754-2754	0.500000	57.4
ndhF_p5	ndhF	5	GGACCAATATGCTTTCGCCT	TGGACCGCGAGAGTTATTTG	20	20	50.0	50.0	58.3	58.0	0.0	8.2	0.324245	117620	119887	2740-2740	0.500000	57.4
ycf1_p1	ycf1	1	ATCCTCAAATGATTCCCGCC	GCCCGACAAAATCTTTCGTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.049371	128891	129010	1057-1057	0.500000	59.6
ycf1_p2	ycf1	2	GCAGCTTAGAACCAGTACCC	GCCCGACAAAATCTTTCGTG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.061842	128891	129010	541-541	0.500000	59.5
ycf1_p3	ycf1	3	GGCAGCTTAGAACCAGTACC	GCCCGACAAAATCTTTCGTG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.061842	128891	129010	542-542	0.500000	59.5
ycf1_p4	ycf1	4	GGGCAGCTTAGAACCAGTAC	GCCCGACAAAATCTTTCGTG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.061842	128891	129010	543-543	0.500000	59.5
ycf1_p5	ycf1	5	ATCCTCAAATGATTCCCGCC	GAGAAAGCAAGCCCGAAAAC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.064996	128891	129010	994-994	0.500000	59.5
ycf1-trnN-GUU_p1	ycf1-trnN-GUU	1	GCTGAAACTGCGCATTGAAT	AACCTACACCGAGGGATTGA	20	20	45.0	50.0	58.0	58.1	12.2	0.0	0.066318	133973	134443	1034-1687	1.000000	91.5
ycf1-trnN-GUU_p2	ycf1-trnN-GUU	2	GCTGAAACTGCGCATTGAAT	GCCTGTAGCTCAGAGGAGTA	20	20	45.0	55.0	58.0	57.9	12.2	12.9	0.073352	133973	134443	1134-1787	1.000000	91.4
ycf1-trnN-GUU_p3	ycf1-trnN-GUU	3	GAGTGCGGTCAGAAATCCAT	CCTCGCAATCACTAGCCAAT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.070797	133973	134443	1284-2290	1.000000	90.8
ycf1-trnN-GUU_p4	ycf1-trnN-GUU	4	GCTGAAACTGCGCATTGAAT	GGTGACGATACTGTAGGGGA	20	20	45.0	55.0	58.0	57.9	12.2	0.0	0.076273	133973	134443	1448-2454	1.000000	90.2
ycf1-trnN-GUU_p5	ycf1-trnN-GUU	5	GCTGAAACTGCGCATTGAAT	CCTCGCAATCACTAGCCAAT	20	20	45.0	50.0	58.0	58.0	12.2	0.0	0.054260	133973	134443	1575-2581	1.000000	90.0