Hotspot

rpl16

LSC · 80383-81813 · 1431 bp

Back to Saxifraga

Pi 0.0452

MarkerSeek score 72.40

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1431
variable_sites: 456
pi: 0.0452
unique_haplotype_count: 101
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CGCACCAAAAACTCGAGTTC	ATCAGAGAAGGGAGGGTTCC	50.0	55.0	58.0	58.1	1848-2288 bp	0.991379	90.6
rpl16_p2	CGCACCAAAAACTCGAGTTC	GGATCAGAGAAGGGAGGGTT	50.0	55.0	58.0	58.1	1850-2290 bp	0.991379	90.6
rpl16_p3	CGCACCAAAAACTCGAGTTC	ATGGATCAGAGAAGGGAGGG	50.0	55.0	58.0	57.9	1852-2292 bp	0.982759	90.2
rpl16_p4	CGCACCAAAAACTCGAGTTC	TCTGGTGTTGAGGGAATTGC	50.0	50.0	58.0	58.1	2220-2591 bp	0.922414	88.0
rpl16_p5	CGCACCAAAAACTCGAGTTC	CCTACAAACGATTCGAGCGA	50.0	50.0	58.0	58.1	1828-2268 bp	0.922414	88.0