Hotspot

rpl32-trnL-UAG

SSC · 112041-112532 · 492 bp

Back to Saxifraga

Pi 0.0876
MarkerSeek score 63.90
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
492
variable_sites
220
pi
0.0876
unique_haplotype_count
85
primer_available
yes
alignment_reliability
0.62
markerseek_score
63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGATATTGGGCAGCGTTAA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.0 58.0 401-643 bp 1.0 91.7
rpl32-trnL-UAG_p2 GGGATATTGGGCAGCGTTAA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.0 58.0 402-644 bp 1.0 91.7
rpl32-trnL-UAG_p3 ATATTGGGCAGCGTTAAGGG AGCGTGTCTACCGATTTCAC 50.0 50.0 58.0 58.0 564-632 bp 0.12069 56.6
rpl32-trnL-UAG_p4 ATATTGGGCAGCGTTAAGGG CAGCGTGTCTACCGATTTCA 50.0 50.0 58.0 58.0 565-633 bp 0.12069 56.6
rpl32-trnL-UAG_p5 ATATTGGGCAGCGTTAAGGG GTGCTCCGATTTCATCCACT 50.0 50.0 58.0 58.0 840-913 bp 0.12069 56.4