Hotspot

petA-psbJ

LSC · 62733-63300 · 568 bp

Back to Saxifraga

Pi 0.0674

MarkerSeek score 80.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 568
variable_sites: 221
pi: 0.0674
unique_haplotype_count: 92
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCATCTGTTATTTTGGCACA	TGGTTCATATTCAGGATTGGGT	42.9	40.9	57.4	57.1	632-738 bp	0.991379	55.9
petA-psbJ_p2	GGCATCTGTTATTTTGGCACA	ATTCAGGATTGGGTTCATCC	42.9	45.0	57.4	55.1	624-730 bp	1.0	55.7
petA-psbJ_p3	TGGCATCTGTTATTTTGGCAC	TGGTTCATATTCAGGATTGGGT	42.9	40.9	57.4	57.1	633-715 bp	0.974138	55.2
petA-psbJ_p4	TGGCATCTGTTATTTTGGCAC	ATTCAGGATTGGGTTCATCC	42.9	45.0	57.4	55.1	625-707 bp	0.982759	55.0
petA-psbJ_p5	GGCATCTGTTATTTTGGCACA	TGGTTCATATTCAGGATTGGGTT	42.9	39.1	57.4	57.8	632-738 bp	0.991379	53.3