Hotspot

rbcL-accD

LSC · 56585-57746 · 1162 bp

Back to Saxifraga

Pi 0.0334

MarkerSeek score 74.90

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 1162
variable_sites: 351
pi: 0.0334
unique_haplotype_count: 99
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	AGTCCTGAATTAGCTGCTGC	TGCAACTATTAATATGATTACTCCAAC	50.0	29.6	58.0	56.1	1045-1361 bp	1.0	52.0
rbcL-accD_p2	AGTCCTGAATTAGCTGCTGC	GCAACTATTAATATGATTACTCCAACT	50.0	29.6	58.0	55.9	1044-1360 bp	1.0	52.0
rbcL-accD_p3	AGTCCTGAATTAGCTGCTGC	TGCAACTATTAATATGATTACTCCAA	50.0	26.9	58.0	54.9	1045-1361 bp	1.0	52.0
rbcL-accD_p4	AGTCCTGAATTAGCTGCTGC	TGCAACTATTAATATGATTACTCCA	50.0	28.0	58.0	54.2	1045-1361 bp	0.931034	49.2
rbcL-accD_p5	AGTCCTGAATTAGCTGCTGC	ATGCAACTATTAATATGATTACTCCA	50.0	26.9	58.0	54.7	1046-1362 bp	0.931034	49.2