Hotspot

psaA-ycf3

LSC · 41762-42373 · 612 bp

Back to Saxifraga

Pi 0.0438

MarkerSeek score 74.90

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 612
variable_sites: 196
pi: 0.0438
unique_haplotype_count: 90
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CTTCTGGTTCCGGCGAAC	TTGAAGATCACGAGGCGTTT	61.1	45.0	58.4	57.8	480-710 bp	0.991379	70.8
psaA-ycf3_p2	CTTCTGGTTCCGGCGAAC	GTTCGATCAAGCTGCTGAGT	61.1	50.0	58.4	58.3	556-786 bp	1.0	70.3
psaA-ycf3_p3	CTTCTGGTTCCGGCGAAC	TGAAGATCACGAGGCGTTTC	61.1	50.0	58.4	58.3	479-709 bp	0.991379	69.8
psaA-ycf3_p4	CTTCTGGTTCCGGCGAAC	TTGGTTGAAGATCACGAGGC	61.1	50.0	58.4	58.2	484-707 bp	0.956897	69.2
psaA-ycf3_p5	CTTCTGGTTCCGGCGAAC	CTTGGTTCGATCAAGCTGCT	61.1	50.0	58.4	58.6	560-790 bp	1.0	68.1