Hotspot

trnE-UUC-trnT-GGU

LSC · 30459-31019 · 561 bp

Back to Saxifraga

Pi 0.0507

MarkerSeek score 76.80

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 561
variable_sites: 205
pi: 0.0507
unique_haplotype_count: 87
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	445-688 bp	1.0	89.8
trnE-UUC-trnT-GGU_p2	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	424-667 bp	1.0	89.7
trnE-UUC-trnT-GGU_p3	GCTGCCTCCTTGAAAGAGAG	CCGATGACTTACGCCTTACC	55.0	55.0	58.3	58.2	442-685 bp	1.0	88.7
trnE-UUC-trnT-GGU_p4	GCTGCCTCCTTGAAAGAGAG	TACCGCTGAGTTAAAAGGGC	55.0	50.0	58.3	57.9	410-653 bp	0.991379	88.7
trnE-UUC-trnT-GGU_p5	TGTCCTGAACCACTAGACGA	TACCGCTGAGTTAAAAGGGC	50.0	50.0	57.7	57.9	389-632 bp	0.991379	88.6