Hotspot

atpH-atpI

LSC · 13593-14487 · 895 bp

Back to Saxifraga

Pi 0.047

MarkerSeek score 88.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 895
variable_sites: 318
pi: 0.047
unique_haplotype_count: 100
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 88.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	598-1028 bp	0.939655	88.2
atpH-atpI_p2	AGCGGCAGAAATCAATGGAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	57.9	57.9	590-1020 bp	0.939655	88.0
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.9	57.9	654-1084 bp	0.939655	87.6
atpH-atpI_p4	AAGCGGCAGAAATCAATGGA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.2	57.9	591-1021 bp	0.939655	87.2
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.2	57.9	599-1029 bp	0.939655	87.1